Katarina Šimunović (Avtor), Orhan Sahin (Avtor), Andi Erega (Avtor), Polonca Štefanič (Avtor), Qijing Zhang (Avtor), Ines Mandić-Mulec (Avtor), Sonja Smole Možina (Avtor), Anja Klančnik (Avtor)

Povzetek

Campylobacter jejuni is the leading cause of bacterial gastroenteritis, or campylobacteriosis, in humans worldwide, and poultry serves as a major source of infection. To reduce the risk associated with C. jejuni transmission via poultry meat, effective interventions during poultry production are needed, and the use of probiotics is a promising approach. In this study, 15 Bacillus subtilis strains were initially screened for their anti-Campylobacter activities. B. subtilis PS-216 strain demonstrated the best anti-Campylobacter activity against 15 C. jejuni isolates when examined using in vitro co-cultures. To evaluate the suitability of B. subtilis PS-216 for probiotic use, its susceptibility to eight clinically important antimicrobials and simulated gastric conditions was investigated. B. subtilis PS-216 was sensitive to all of the tested antibiotics. Although vegetative cells were sensitive to gastric conditions, B. subtilis PS-216 spores were highly resistant. We further evaluated the use of a B. subtilis PS-216 spore preparation (2.5 × 106 CFU/mL water) to prevent and/or reduce C. jejuni colonization in broiler chickens in vivo. Compared to the untreated group, significantly lower Campylobacter counts were detected in caeca of broilers continuously treated with B. subtilis PS-216 spores in their drinking water. Furthermore, broilers continuously treated with B. subtilis PS-216 spores showed improved weight gain, compared to the control group. Together, these results demonstrate the potential of B. subtilis PS-216 for use in poultry to reduce C. jejuni colonization and improve weight gain.

Ključne besede

probiotiki;inhibicija rasti;sporogene bakterije;perutnina;Campylobacter jejuni;Bacillus subtilis;probiotics;alternative to antibiotics;spore-containing drinking water;broiler chicken;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL BF - Biotehniška fakulteta
UDK: 579.22/.26:579.67
COBISS: 115015683 Povezava se bo odprla v novem oknu
ISSN: 1664-302X
Št. ogledov: 101
Št. prenosov: 32
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: probiotiki;inhibicija rasti;sporogene bakterije;perutnina;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-11
Zvezek: ǂVol. ǂ13
Čas izdaje: Jul. 2022
DOI: 10.3389/fmicb.2022.910616
ID: 18625363