diplomsko delo
Luka Irenej Pečan (Author), Jana Murovec (Mentor), Barbara Jeršek (Co-mentor)

Abstract

Namen diplomskega dela je bil ovrednotiti protibakterijski in protiadhezivni učinek ekstraktov štirih različnih kemotipov konoplje (Cannabis sativa) na različne seve bakterij vrst S. aureus in S. epidermidis. Z metodo razredčevanja v mikrotitrski ploščici (MTP) smo določili minimalno inhibitorno koncentracijo (MIK), potrdili smo jo tudi z metodo štetja kolonij na trdnem gojišču. Ekstrakti konoplje so pokazali različne vrednosti MIK pri posameznih sevih v razponu od 2,0±0 µl/mL do 10,4±2,12 µl/mL, prav tako pa je posamezen ekstrakt na izbrane seve deloval različno. Skladno z določenimi vrednostmi MIK smo določili protiadhezivno delovanje štirih ekstraktov konoplje za dva tipska seva bakterij vrste S. epidermidis. Za preverjanje učinkovanja ekstraktov na pritrjanje celic na mikrotitrsko ploščico smo uporabili metodo barvanja s kristal vijoličnim. Testirali smo koncentracije ekstraktov v vrednosti 0,5 MIK in 0,1 MIK. Protiadhezivno delovanje smo določili pri E2 in MIK 0,1, E3 in MIK 0,5, E4 in MIK 0,5, E5 in MIK 0,1 za sev S. epidermidis ŽMJ 489 ne pa tudi za sev S. epidermidis ŽMJ 465. Glede na rezultate lahko zaključimo, da imajo kanabinoidi v farmaciji ali medicini potencial v boju proti rezistentnim sevom patogenih mikroorganizmov, kljub temu pa je treba opozoriti, da so raziskave na tem področju še vedno v začetni fazi.

Keywords

ekstrakt konoplje;protimikrobno delovanje;protiadhezivno delovanje;minimalna inhibitorna koncentracija;

Data

Language: Slovenian
Year of publishing:
Typology: 2.11 - Undergraduate Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [L. I. Pečan]
UDC: 606:61:582.630.1:543.645.7(043.2)
COBISS: 162537987 Link will open in a new window
Views: 24
Downloads: 6
Average score: 0 (0 votes)
Metadata: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Other data

Secondary language: English
Secondary title: Antibacterial and anti-adhesive activity of hemp (Cannabis sativa) extracts
Secondary abstract: The aim of the thesis was to evaluate the effect of extracts of four different cannabis chemotypes on different strains of S. aureus and S. epidermidis. The minimum inhibitory concentration (MIC) was determined by microdilution method and confirmed by the colony counting method on a solid medium. Cannabis extracts showed different MIC on individual strains, ranging from a minimum of 2.0±0 µl/mL to a maximum of 10.4±2.12 µl/mL, individual extracts had different effects on selected strains. In accordance with the established MICs, we aimed to determine the anti-adhesive activity of the four extracts on two of S. epidermidis type strains. We used the crystal violet staining method to verify the effect of the extracts on cell attachment to the microtitre plate. Concentrations of 0.5 MIC and 0.1 MIC were tested. The anti-adhesive activity was determined for E2 and MIC 0,1, E3 and MIC 0,5, E4 and MIC 0,5, E5 and MIC 0,1 for S. epidermidis ŽMJ 489 strain but not for S. epidermidis ŽMJ 465 strain. In view of the results, it can be concluded that cannabinoids have potential in pharmaceutical or medical applications to combat resistant strains of pathogenic microorganisms, although it should be noted that research in this field is still at an early stage.
Secondary keywords: cannabis extract;antimicrobial activity;pro-adhesive activity;minimal inhibitory concentration;
Type (COBISS): Bachelor thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: 1 spletni vir (1 datoteka PDF (VIII, 20 str., [3] str. pril.))
ID: 19853828