magistrsko delo
Doroteja Krajnc (Author), Aleš Podgornik (Mentor), Marjan Marinšek (Thesis defence commission member), Gabriela Kalčíková (Thesis defence commission member)

Abstract

Bakteriofagi so virusi, ki ne obstajajo in se ne morejo razmnoževati brez bakterijske gostiteljske celice. Ob tem je končni rezultat liza bakterijske celice, zato so prepoznani kot orodje v boju proti bakterijskim infekcijam. Pregled literature kaže na široko rabo bakteriofagov v prehrambeni industriji in kmetijstvu. Zaradi naraščujoče odpornosti bakterij na antibiotike je to potencialna rešitev tudi na področju medicine, natančneje pri uporabi na človeku. Zahteva po bakteriofagih zato narašča, posledično narašča potreba po optimizaciji proizvodnih procesov, razumevanje interakcije fag-bakterija pa je ključ načrtovanja le-teh. V tej luči so bili izvedeni eksperimenti na sistemu fag K- Staphylococcus capitis v šaržnih in kontinuirnih procesih, skozi katere smo opazovali latentno periodo, adsorpcijsko konstanto, pomnožitveno število in eklipsno periodo pri različnih specifičnih hitrostih rasti bakterijskih celic oziroma hitrostih redčenja. Fiziologija bakterijskih celic je namreč neposredno povezana z omenjenimi parametri. Pri naraščujoči specifični hitrosti rasti oziroma hitrosti redčenja smo v obeh sistemih opazili naračujočo adsorpcijsko konstanto ter pomnožitveno število. Latentna perioda in eklipsna perioda pri istem trendu specifične hitrosti rasti oziroma hitrosti redčenja padata v šaržnih sistemih, medtem ko za kontinuirne sisteme nismo pridobili zadostnih podatkov.

Keywords

stafilokoki;Staphylococcus capitis;gojenje mikroorganizmov;šaržni sistem;kontinuirni sistem;rastni parametri;magistrska dela;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL FKKT - Faculty of Chemistry and Chemical Technology
Publisher: [D. Krajnc]
UDC: 602.3:578.347(043.2)
COBISS: 164001283 Link will open in a new window
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Downloads: 0
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Other data

Secondary language: English
Secondary title: The effect of bacteriophages on Staphylococci in various physiological states
Secondary abstract: Bacteriophages are viruses that cannot exist and reproduce without a bacterial host cell. The end result is bacterial cell lysis, which means bacteriophages can be used as a fighting tool against bacterial infections. Literature shows the widespread use of bacteriophages in the food industry and agro-culture. The increasing bacterial resistance to antibiotics also makes it a potential solution in the field of medicine, more specifically human applications. Demand for bacteriophages is therefore increasing, and so is the need of optimal production processes. Understanding phage-bacteria interaction is key, therefore, experiments were carried out on the phage K- Staphylococcus capitis system in batch and continuous reactors. Since physiology of bacterial cells is directly linked to these parameters, latent period, adsorption constant, burst size and eclipse period were observed at different specific bacterial cell growth and dilution rates, respectively. At increasing specific growth rate and dilution rate, an increasing adsorption constant and burst size were observed in both systems. The decrease of latent period and eclipse period for the same trend in specific growth rate and dilution rate was observed in batch reactors, while for continuous reactors the data was insufficient.
Secondary keywords: bacteriophages;staphylococci;batch reactors;continuous reactors;Bakteriofagi;Univerzitetna in visokošolska dela;
Type (COBISS): Master's thesis/paper
Study programme: 1000376
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Fak. za kemijo in kemijsko tehnologijo, smer Kemijsko inženirstvo
Pages: 62 str.
ID: 19908960