Spektroskopske raziskave kemijske denaturacije lizocima
diplomsko delo
Abstract
Namen diplomske naloge je bil preučiti, kako dodatek gvanidin hidroklorida (GdHCl) vpliva na kemijsko denaturacijo proteina lizocima pri treh različnih temperaturah. Sam potek kemijske denaturacije lizocima sem spremljala z merjenjem fluorescence. Na podlagi meritev sem izračunala stopnjo denaturacije lizocima in standardno prosto Gibbsovo energijo ter standardno entalpijo za denaturacijo lizocima. Vrednosti sem primerjala z vrednostmi, pridobljenih iz toplotne denaturacije ter literature.
Keywords
proteini;denaturacija proteinov;gvanidin hidroklorid;fluorescenca;fluorescenčna spektroskopija;diplomske naloge;
Data
| Language: | Slovenian |
|---|---|
| Year of publishing: | 2023 |
| Typology: | 2.11 - Undergraduate Thesis |
| Organization: | UL FKKT - Faculty of Chemistry and Chemical Technology |
| Publisher: | [L. Martinčič] |
| UDC: | 577.322:543.42(043.2) |
| COBISS: |
164550147
|
| Views: | 17 |
| Downloads: | 2 |
| Average score: | 0 (0 votes) |
| Metadata: |
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Other data
| Secondary language: | English |
|---|---|
| Secondary title: | Spectroscopic research of chemical denaturation of lysozyme |
| Secondary abstract: | The aim of the thesis was to examine how the addition of guanidine hydrochloride (GdHCl) affects the chemical denaturation of lysozyme protein at three different temperatures. The progress of lysozyme's chemical denaturation was monitored by measuring fluorescence. Based on the measurements, I calculated the degree of lysozyme denaturation and the standard Gibbs energy and standard enthalpy for denaturation of lysozyme. The values were compared to the values obtained from thermal denaturation and literature. |
| Secondary keywords: | proteins;lysozyme;guanidine hydrochloride;fluorescence;denaturation;Lizocim;Univerzitetna in visokošolska dela; |
| Type (COBISS): | Bachelor thesis/paper |
| Study programme: | 1000374 |
| Embargo end date (OpenAIRE): | 1970-01-01 |
| Thesis comment: | Univ. v Ljubljani, Fak. za kemijo in kemijsko tehnologijo, VSŠ Kemijska tehnologija |
| Pages: | 32 str. |
| ID: | 19908999 |
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