Vpliv nima (azadirachta indica a. juss) in triphale mešanice na preživetje človeških melanomskih celic in keratinocitov v celični kulturi

magistrsko delo

Ana Marija Hirtl (Author), Saška Lipovšek Delakorda (Mentor), Barbara Dariš (Co-mentor), Biljana Dušić (Co-mentor)

Abstract

Nim in triphala mešanica (v nadaljevanju triphala) sta v ajurvedski medicini uporabljeni za lajšanje različnih bolezenskih stanj. V magistrskem delu smo želeli raziskati vpliv nima in triphale na obliko in metabolno aktivnost melanomskih celic WM 266-4 in keratinocitov NHEK. S tem namenom smo pripravili gojišči za celični kulturi melanomskih celic WM 266-4 in keratinocitov NHEK, gojili celice in analizirali vplive raztopin nima in triphale na opazovane celice. Pri eksperimentalnem delu smo uporabili metodi svetlobne mikroskopije in spektrofotometrije. S slednjo smo analizirali rezultate testa WST8, ki je metoda za določitev metabolne aktivnosti. Ugotovili smo, da nim vpliva na obliko melanomskih celic WM 266-4 in zmanjša njihovo metabolno aktivnost. Nim ni vplival na obliko in metabolno aktivnost keratinocitov NHEK. Triphala ni vplivala na obliko melanomskih celic WM 266-4; nizke koncentracije triphale so njihovo metabolno aktivnost zmanjšale, najvišja koncentracija jo je povečala. Triphala ni vplivala na obliko keratinocitov NHEK; njihova metabolna aktivnost se je povečala pri vseh koncentracijah triphale. Raztopine nima in triphale skupaj so povzročile spremembo oblike melanomskih celic WM 266-4 in zmanjšale njihovo metabolno aktivnost. Raztopine nima in triphale skupaj so vplivale na obliko nekaterih keratinocitov NHEK. Metabolna aktivnost keratinocitov NHEK je bila povečana pri najvišji koncentraciji nima in triphale; nižje koncentracije nima in triphale niso vplivale na njihovo metabolno aktivnost. Ker so vse raztopine nima vplivale na obliko melanomskih celic WM 266-4 in zmanjšale njihovo metabolno aktivnost, bi bilo smiselno nadaljevati z raziskavami njegovih učinkov na različne tipe celic.

Keywords

magistrska dela;nim;triphala;melanom;melanomske celice in keratinociti;

Data

Language:	Slovenian
Year of publishing:	2023
Typology:	2.09 - Master's Thesis
Organization:	UM FNM - Faculty of Natural Sciences and Mathematics
Publisher:	[A. M. Hirtl]
UDC:	616-006.81:57/59(043.2)
COBISS:	167170307
Views:	8
Downloads:	1
Average score:	0 (0 votes)
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Other data

Secondary language:	English
Secondary title:	The influence of neem (azadirachta indica a. juss) and triphala mix on the survival of human melanoma cells and keratinocytes in the cell culture
Secondary abstract:	The neem and triphala mix (hereinafter referred to as triphala) are used in Ayurvedic medicine to alleviate various health conditions. The aim of this master thesis was to investigate the impact of neem and triphala on the morphology and metabolic activity of WM 266-4 melanoma cells and NHEK keratinocytes. For this purpose, a culture media for WM 266-4 melanoma cells and NHEK keratinocytes was prepared. The cells were cultured, and the effects of neem and triphala solutions on the observed cells were analysed. The experimental work was carried out using light microscopy and spectrophotometry. The latter was used for the analysis of the WST8 test, which is a method for determining metabolic activity. Neem affected the morphology of WM 266-4 melanoma cells and reduced their metabolic activity. Neem did not affect the morphology and metabolic activity of NHEK keratinocytes. Triphala did not affect the morphology of WM 266-4 melanoma cells; low concentrations of triphala reduced their metabolic activity, while the highest concentration increased it. Triphala did not affect the morphology of NHEK keratinocytes; their metabolic activity increased at all concentrations of triphala. Neem and triphala together caused a change in the shape of WM 266-4 melanoma cells and reduced their metabolic activity. Neem and triphala together affected the morphology of some NHEK keratinocytes. The metabolic activity of NHEK keratinocytes increased at the highest concentration of neem and triphala together; lower concentrations of neem and triphala together did not affect their metabolic activity. Since all neem solutions affected the morphology of WM 266-4 melanoma cells and reduced their metabolic activity, neem deserves further attention in studying its effects on different cell types.
Secondary keywords:	master theses;neem;triphala;melanoma;melanoma cells;keratinocytes;Melanom;Univerzitetna in visokošolska dela;
Type (COBISS):	Master's thesis/paper
Thesis comment:	Univ. v Mariboru, Fak. za naravoslovje in matematiko, Oddelek za biologijo
Pages:	XII, 68 f.
ID:	19980007