magistrsko delo
Simon Ekselenski (Author), Polona Jamnik (Mentor), Mojca Korošec (Thesis defence commission member)

Abstract

Za biomaso žuželk je značilno, da je bogata s proteini, vsebuje pa tudi številna druga esencialna hranila. Zaradi ugodne hranilne sestave in relativno poceni načina gojenja postajajo žuželke zanimive pri iskanju novih alternativnih virov hranil. Z mlečnokislinsko fermentacijo bi lahko biomaso žuželk obogatili še dodatno ter povečali njeno hranilno vrednost. Kompleksne molekule se tekom fermentacije pretvorijo v enostavnejše in biološko dostopnejše molekule, kar daje končnemu proizvodu vrsto dokazanih pozitivnih učinkov. Suhi biomasi žuželk (substrat), velikega mokarja in hišnega črička, smo izpostavili trem pogojem mlečnokislinske fermentacije: spontani fermentaciji, fermentaciji z dodanim inokulumom L. plantarum ter fermentaciji s komercialno mesno kulturo. Vzorce smo fermentirali 48 h, analizirali pa smo jih pri treh različnih časih (0, 24 in 48 h). Z različnimi analizami fermentiranih proizvodov (določanje kolonijskih enot, merjenje pH, določanje koncentracije mlečne kisline in preverjanje proteinske hidrolize z določanjem koncentracije prostih amino skupin ter s SDS-PAGE) smo spremljali dinamiko fermentacije med vzorci ter ovrednotili nastale produkte. Ugotovili smo, da je fermentacija potekala najbolj izrazito prvih 24 h. Največje povečanje koncentracij končnih produktov (mlečne kisline in prostih amino skupin) smo tekom fermentacije izmerili v vzorcih z inokulumom bakterije L. plantarum, ki je na obeh substratih rasla enako dobro.

Keywords

žuželčja biomasa;veliki mokar;hišni čriček;mlečnokislinska fermentacija;spontana fermentacija;komercialna mesna kultura;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UL BF - Biotechnical Faculty
Publisher: [S. Ekselenski]
UDC: 604.4:664:637.136.5:595.71/.79(043.2)
COBISS: 166619139 Link will open in a new window
Views: 22
Downloads: 6
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Other data

Secondary language: English
Secondary title: Lactic acid fermentation of insects as an alternative protein source
Secondary abstract: Insect biomass is characterized by rich protein content while it also contains many other essential nutrients. Due to favorable nutritional properties and relatively cheap methods of cultivation, insects are becoming an interesting topic in the search for new alternative sources of nutrients. With lactic acid fermentation we could enrich insect biomass even further and increase its nutritional value. During fermentation complex molecules are converted into simpler and biologically more accessible molecules, giving the final product a range of proven positive effects. Two lyophilized biomasses of insects, a mealworm and a house cricket, were exposed to three conditions of lactic acid fermentation: spontaneous fermentation, fermentation with L. plantarum inoculum and fermentation with a commercial meat culture. The samples were fermented for 48 h and analyzed at three different times (0, 24 and 48 h). Through various analyses of fermented products (determining the colony forming units, pH measurement, determining the concentration of lactic acid and verification of protein hydrolysis by determining the concentrations of free amino groups and by SDS-PAGE) we monitored the dynamics of fermentation between samples and evaluated the end products. We found that fermentation was most pronounced in the first 24 h. The greatest increase in the concentration of fermentation end products (lactic acid and free amino groups) was measured in samples during fermentation with L. plantarum inoculum, which grew equally well on both substrates.
Secondary keywords: Lactobacillus plantarum;
Type (COBISS): Master's thesis/paper
Study programme: 0
Embargo end date (OpenAIRE): 1970-01-01
Thesis comment: Univ. v Ljubljani, Biotehniška fak., Študij biotehnologije
Pages: 1 spletni vir (1 datoteka PDF (XII, 41 str., [10] str. pril.))
ID: 20015585