Mojca Škerget (Author), Maja Čolnik (Author), Lidija Fras Zemljič (Author), Lidija Gradišnik (Author), Tanja Živković Semren (Author), Blanka Tariba Lovaković (Author), Uroš Maver (Author)

Abstract

The isolation of keratin from poultry feathers using subcritical water was studied in a batch reactor at temperatures (120–250 °C) and reaction times (5–75 min). The hydrolyzed product was characterized by FTIR and elemental analysis, while the molecular weight of the isolated product was determined by SDS-PAGE electrophoresis. To determine whether disulfide bond cleavage was followed by depolymerization of protein molecules to amino acids, the concentration of 27 amino acids in the hydrolysate was analyzed by GC/MS. The optimal operating parameters for obtaining a high molecular weight protein hydrolysate from poultry feathers were 180 °C and 60 min. The molecular weight of the protein hydrolysate obtained under optimal conditions ranged from 4.5 to 12 kDa, and the content of amino acids in the dried product was low (2.53% w/w). Elemental and FTIR analyses of unprocessed feathers and dried hydrolysate obtained under optimal conditions showed no significant differences in protein content and structure. Obtained hydrolysate is a colloidal solution with a tendency for particle agglomeration. Finally, a positive influence on skin fibroblast viability was observed for the hydrolysate obtained under optimal processing conditions for concentrations below 6.25 mg/mL, which makes the product interesting for various biomedical applications.

Keywords

subkritična hidroliza vode;fizikalno kemijske lastnosti;citotoksičnost;poultry feathers;subcritical water hydrolysis;keratin;physico-chemical characterization;cytotoxicity;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UM MF - Faculty of Medicine
Publisher: MDPI
UDC: 66.02
COBISS: 155799299 Link will open in a new window
ISSN: 2073-4360
Views: 221
Downloads: 11
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Other data

Secondary language: Slovenian
Secondary keywords: subkritična hidroliza vode;fizikalno kemijske lastnosti;citotoksičnost;
Type (COBISS): Article
Pages: 15 str.
Volume: ǂVol. ǂ15
Issue: ǂiss. ǂ12, [article no.] 2658
Chronology: June 2023
DOI: 10.3390/polym15122658
ID: 21303844