Mojca Škerget (Avtor), Maja Čolnik (Avtor), Lidija Fras Zemljič (Avtor), Lidija Gradišnik (Avtor), Tanja Živković Semren (Avtor), Blanka Tariba Lovaković (Avtor), Uroš Maver (Avtor)

Povzetek

The isolation of keratin from poultry feathers using subcritical water was studied in a batch reactor at temperatures (120–250 °C) and reaction times (5–75 min). The hydrolyzed product was characterized by FTIR and elemental analysis, while the molecular weight of the isolated product was determined by SDS-PAGE electrophoresis. To determine whether disulfide bond cleavage was followed by depolymerization of protein molecules to amino acids, the concentration of 27 amino acids in the hydrolysate was analyzed by GC/MS. The optimal operating parameters for obtaining a high molecular weight protein hydrolysate from poultry feathers were 180 °C and 60 min. The molecular weight of the protein hydrolysate obtained under optimal conditions ranged from 4.5 to 12 kDa, and the content of amino acids in the dried product was low (2.53% w/w). Elemental and FTIR analyses of unprocessed feathers and dried hydrolysate obtained under optimal conditions showed no significant differences in protein content and structure. Obtained hydrolysate is a colloidal solution with a tendency for particle agglomeration. Finally, a positive influence on skin fibroblast viability was observed for the hydrolysate obtained under optimal processing conditions for concentrations below 6.25 mg/mL, which makes the product interesting for various biomedical applications.

Ključne besede

subkritična hidroliza vode;fizikalno kemijske lastnosti;citotoksičnost;poultry feathers;subcritical water hydrolysis;keratin;physico-chemical characterization;cytotoxicity;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UM MF - Medicinska fakulteta
Založnik: MDPI
UDK: 66.02
COBISS: 155799299 Povezava se bo odprla v novem oknu
ISSN: 2073-4360
Št. ogledov: 221
Št. prenosov: 11
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: subkritična hidroliza vode;fizikalno kemijske lastnosti;citotoksičnost;
Vrsta dela (COBISS): Članek v reviji
Strani: 15 str.
Letnik: ǂVol. ǂ15
Zvezek: ǂiss. ǂ12, [article no.] 2658
Čas izdaje: June 2023
DOI: 10.3390/polym15122658
ID: 21303844