ǂthe ǂinfluence of venepuncture and short blood storage
Ivica Marić (Author), Klemen Žiberna (Author), Ana Kolenc (Author), Elvira Maličev (Author)

Abstract

Extracellular vesicles (EVs) as membrane-bound particles released by various cells are potential tools for diagnosis and treatment. Blood cells, particularly platelets, are the source of circulating EVs. Material: EVs were enriched with gradient ultracentrifugation and measured by nanoparticle tracking assay. A flow cytometric multiplex assay was used for cellular source determination. Activation of platelets was measured as a percentage of CD62p+/CD61+ platelets and correlated with the concentration and size of released EVs. Results: In general there was no statistically significant correlation between EVs` concentration and degree of platelet activation. EVs from different cellular sources were detected. Comparing different needle thicknesses, there was a decrease in the EVs concentration for the 16G needle versus the 21G needle, but no difference was observed for EVs` size and phenotype or platelets activation. During blood storage, platelet activation increased, but there was no effect on the EVs` concentration, size, or phenotype. Conclusions: Preanalytical factors like needle thickness and storage time can affect the MVs' properties. Activation of platelets during blood collection or blood storage occurs; however, it is difficult to determine its effect on the physiological properties of EVs since the mechanisms of EVs` biogenesis and especially clearness are not precisely known.

Keywords

blood;blood plasma;extracellular vesicles;flow cytometry;platelet activation;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UL MF - Faculty of Medicine
UDC: 616.1
COBISS: 192381699 Link will open in a new window
ISSN: 1079-9796
Views: 292
Downloads: 165
Average score: 0 (0 votes)
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Other data

Secondary language: Slovenian
Secondary keywords: kri;krvna plazma;zunajcelični vezikli;pretočna citometrija;aktivacija trombocitov;
Type (COBISS): Article
Pages: str. 1-7
Volume: ǂVol. ǂ106
Issue: ǂiss. ǂ[article no.] 102842
Chronology: 2024
DOI: 10.1016/j.bcmd.2024.102842
ID: 23688022