Darja Božič (Avtor), Simona Prelesnik (Avtor), Ita Junkar (Avtor), Roman Štukelj (Avtor), Manca Pajnič (Avtor), Ema Žagar (Avtor), Veronika Kralj-Iglič (Avtor), Ksenija Kogej (Avtor)

Povzetek

Extracellular vesicles (EVs) isolated from biological samples are a promising material for use in medicine and technology. However, the assessment methods that would yield repeatable concentrations, sizes and compositions of the harvested material are missing. A plausible model for the description of EV isolates has not been developed. Furthermore, the identity and genesis of EVs are still obscure and the relevant parameters have not yet been identified. The purpose of this work is to better understand the mechanisms taking place during harvesting of EVs, in particular the role of viscosity of EV suspension. The EVs were harvested from blood plasma by repeated centrifugation and washing of samples. Their size and shape were assessed by using a combination of static and dynamic light scattering. The average shape parameter of the assessed particles was found to be ρ ~ 1 (0.94–1.1 in exosome standards and 0.7–1.2 in blood plasma and EV isolates), pertaining to spherical shells (spherical vesicles). This study has estimated the value of the viscosity coefficient of the medium in blood plasma to be 1.2 mPa/s. It can be concluded that light scattering could be a plausible method for the assessment of EVs upon considering that EVs are a dynamic material with a transient identity.

Ključne besede

izvencelični vezikli;eksosomi;krvna plazma;dinamično sipanje svetlobe;statično sipanje svetlobe;določanje oblike;viskoznost krvne plazme;extracellular vesicles;exosomes;blood plasma;dynamic light scattering;static light scattering;shape characterization;viscosity of blood plasma;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL FKKT - Fakulteta za kemijo in kemijsko tehnologijo
UDK: 577.35
COBISS: 1538312131 Povezava se bo odprla v novem oknu
ISSN: 2073-4409
Št. ogledov: 224
Št. prenosov: 79
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: izvencelični vezikli;eksosomi;krvna plazma;dinamično sipanje svetlobe;statično sipanje svetlobe;določanje oblike;viskoznost krvne plazme;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-21
Letnik: ǂVol. ǂ8
Zvezek: ǂiss. ǂ9
Čas izdaje: Sep. 2019
DOI: 10.3390/cells8091046
ID: 13805879