Jelena Djordjević (Author), Stoimir Kolarević (Author), Jovana Jovanović Marić (Author), Margareta Kračun-Kolarević (Author), Bojana Žegura (Author), Alja Štraser (Author), Dušan M. Sladić (Author), Irena Novaković (Author), Branka Vuković-Gačić (Author)

Abstract

The aim of this study was to investigate the effects of tert-butylquinone (TBQ) and its alkylthio and arylthio derivatives on DNA in vitro, using acellular and cellular test systems. Direct interaction with DNA was studied using the plasmid pUC19. Cytotoxic (MTS assay) and genotoxic (comet assay and γH2AX focus assays) effects, and their influence on the cell cycle were studied in the HepG2 cell line. Our results show that TBQ and its derivatives did not directly interact with DNA. The strongest cytotoxic effect on the HepG2 cells was observed for the derivative 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone (IC50 64.68 and 55.64 μM at 24-h and 48-h treatment, respectively). The tested derivatives did not significantly influence the cell cycle distribution in the exposed cellular populations. However, all derivatives showed a genotoxic activity stronger than that of TBQ in the comet assay, with 2-tert-butyl-5,6-(ethylenedithio)-1,4-benzoquinone producing the strongest effect. The same derivative also induced DNA double-strand breaks in the γH2AX focus assay.

Keywords

TBQ derivatives;HepG2 cell line;comet assay;γH2AX assay;cell cycle analysis;cytotoxicity;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: NIB - National Institute of Biology
UDC: 577.2
COBISS: 200413443 Link will open in a new window
ISSN: 1879-3177
Views: 124
Downloads: 104
Average score: 0 (0 votes)
Metadata: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Other data

Secondary language: Slovenian
Secondary keywords: derivati TBQ;celična linija HepG2;test komet;test gH2AX;analiza celičnega cikla;citotoksičnost;
Source comment: Soavtorji: Stoimir Kolarević, Jovana Jovanović Marić, Margareta Kračun-Kolarević, Bojana Žegura, Alja Štern, Dušan Sladić, Irena Novaković, Branka Vuković-Gačić; Nasl. z nasl. zaslona; Opis vira z dne 2. 7. 2024;
Pages: str. 1-8
Volume: ǂVol. ǂ99
Issue: ǂ[article no.] ǂ105882
Chronology: 2024
DOI: 10.1016/j.tiv.2024.105882
ID: 24506149