Polona Kogovšek (Author), Nataša Mehle (Author), Anja Pugelj (Author), Tjaša Jakomin (Author), Hans-Josef Schroers (Author), Maja Ravnikar (Author), Marina Dermastia (Author)

Abstract

A fluorescence-based real-time loop-mediated isothermal amplification (LAMP) assay for ‘Candidatus Phytoplasama solani’ (Bois noir phytoplasma; BNp) detection was developed and optimised for rapid laboratory and on-site BNp detection. This assay is highly specific, rapid and as sensitive as qPCR. It was validated according to European and Mediterranean Plant Protection Organisation recommendations. In addition, 286 grapevine leaf samples from the 2015 growing season were tested with this new real-time LAMP assay and an assay previously developed for detection of Flavescence dorée phytoplasma (FDp). These LAMP assays for detection of both BNp and FDp used without any DNA extraction step, which is a required step for qPCR analysis, were comparably effective to qPCR, and positive results were obtained in less than 35 min.

Keywords

real-time LAMP;grapevine yellows phytoplasma;validation;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: NIB - National Institute of Biology
UDC: 581.2
COBISS: 4088399 Link will open in a new window
ISSN: 0929-1873
Views: 19
Downloads: 7
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Other data

Pages: str. 75-84
Volume: ǂVol. ǂ148
Issue: ǂiss. ǂ1
Chronology: 2017
DOI: 10.1007/s10658-016-1070-z
ID: 24586414