Polona Kogovšek (Avtor), Nataša Mehle (Avtor), Anja Pugelj (Avtor), Tjaša Jakomin (Avtor), Hans-Josef Schroers (Avtor), Maja Ravnikar (Avtor), Marina Dermastia (Avtor)

Povzetek

A fluorescence-based real-time loop-mediated isothermal amplification (LAMP) assay for ‘Candidatus Phytoplasama solani’ (Bois noir phytoplasma; BNp) detection was developed and optimised for rapid laboratory and on-site BNp detection. This assay is highly specific, rapid and as sensitive as qPCR. It was validated according to European and Mediterranean Plant Protection Organisation recommendations. In addition, 286 grapevine leaf samples from the 2015 growing season were tested with this new real-time LAMP assay and an assay previously developed for detection of Flavescence dorée phytoplasma (FDp). These LAMP assays for detection of both BNp and FDp used without any DNA extraction step, which is a required step for qPCR analysis, were comparably effective to qPCR, and positive results were obtained in less than 35 min.

Ključne besede

real-time LAMP;grapevine yellows phytoplasma;validation;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: NIB - Nacionalni inštitut za biologijo
UDK: 581.2
COBISS: 4088399 Povezava se bo odprla v novem oknu
ISSN: 0929-1873
Št. ogledov: 414
Št. prenosov: 240
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Strani: str. 75-84
Letnik: ǂVol. ǂ148
Zvezek: ǂiss. ǂ1
Čas izdaje: 2017
DOI: 10.1007/s10658-016-1070-z
ID: 24586414