Nastja Sluga (Author), Sandra Postić (Author), Srdjan Sarikas (Author), Ya-Chi Huang (Author), Andraž Stožer (Author), Marjan Rupnik (Author)

Abstract

Cholinergic innervation in the pancreas controls both the release of digestive enzymes to support the intestinal digestion and absorption, as well as insulin release to promote nutrient use in the cells of the body. The effects of muscarinic receptor stimulation are described in detail for endocrine beta cells and exocrine acinar cells separately. Here we describe morphological and functional criteria to separate these two cell types in situ in tissue slices and simultaneously measure their response to ACh stimulation on cytosolic Ca2+ oscillations [Ca2+]c in stimulatory glucose conditions. Our results show that both cell types respond to glucose directly in the concentration range compatible with the glucose transporters they express. The physiological ACh concentration increases the frequency of glucose stimulated [Ca2+]c oscillations in both cell types and synchronizes [Ca2+]c oscillations in acinar cells. The supraphysiological ACh concentration further increases the oscillation frequency on the level of individual beta cells, inhibits the synchronization between these cells, and abolishes oscillatory activity in acinar cells. We discuss possible mechanisms leading to the observed phenomena.

Keywords

pancreas tissue slices;acetylcholine;beta cell;acinar cell;Ca2+ oscillations;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UM MF - Faculty of Medicine
Publisher: MDPI
UDC: 612.349.7
COBISS: 68553731 Link will open in a new window
ISSN: 2073-4409
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Downloads: 8
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Other data

Secondary language: Slovenian
Secondary keywords: tkivne rezine trebušne slinavke;acetilholin;beta celice;acinarne celice;kalcijeve oscilacije;
Type (COBISS): Scientific work
Pages: str. 1-14
Issue: ǂVol.ǂ10
Chronology: 2021
DOI: 10.3390/cells10071580
ID: 25290851