Natural Deep Eutectic Solvent-Based Matrix Solid Phase dispersion (MSPD) extraction for determination of bioactive compounds from sandy everlasting (Helichrysum arenarium L.)

a case of stability study

Milena Ivanović (Author), Peter Krajnc (Author), Aleš Mlinarič (Author), Maša Islamčević Razboršek (Author)

Abstract

In the present study, vortex-assisted matrix solid-phase dispersion (VA-MSPD) extraction was used to isolate the major bioactive compounds from H. arenarium. To reduce the negative environmental impact of the conventionally used organic solvents, four different choline chloride-based natural deep eutectic solvents (NADES) were investigated as possible eluents. The most influential VA-MSPD extraction parameters: stationary phase (adsorbent), adsorbent/sample ratio, vortex time, and volume of extraction solvent were systematically optimized. Ultrasound-assisted extraction with 80% MeOH was used as the standard method for the comparison of results. The stability of the obtained extracts was studied over a period of 0 to 60 days at three different temperatures (−18 ◦C, 4 ◦C, and 25 ◦C). All extracts were evaluated both spectrophotometrically (determination of total phenolic content (TPC) and antioxidant activity by ABTS and FRAP assay) and chromatographically (HPLC-UV). NADES based on choline chloride and lactic acid (ChCl-LA) was selected as the most effective extractant, with a determined TPC value of its extract of 38.34 ± 0.09 mg GA/g DW (27% higher than the methanolic VA-MSPD extract) and high antioxidant activity. The content of individual phenolic compounds (chlorogenic acid, dicaffeoylquinic acid isomers, naringenin isomers, and chalcones) in the ChCl-LA extract, determined by HPLC-UV, was comparable to that of the conventionally obtained one. Moreover, the stabilization effect of ChCl-LA was confirmed for the studied compounds: chlorogenic acid, naringenin-40 -O-glucoside, tomoroside A, naringenin-5-Oglucoside, isosalipurposide, and naringenin. The optimum VA-MSPD conditions for the extraction of H. arenarium polyphenols were: florisil/sample ratio of 0.5/1, a vortex time of 2 min, and an elution volume of ChCl-LA of 10 mL.

Keywords

fenoli;antioksidanti;ekstrakcija;matrix solid phase dispersion extraction;MSPD;natural deep eutectic solvents;NADES;extraction optimization;sandy everlasting;phenolic compounds;stability studies;antioxidant activity;

Data

Language:	English
Year of publishing:	2022
Typology:	1.01 - Original Scientific Article
Organization:	UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher:	MDPI
UDC:	66.02
COBISS:	134436867
ISSN:	2223-7747
Views:	0
Downloads:	0
Average score:	0 (0 votes)
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Other data

Secondary language:	Slovenian
Secondary keywords:	fenoli;antioksidanti;ekstrakcija;
Type (COBISS):	Article
Pages:	17 str.
Volume:	ǂVol. ǂ11
Issue:	ǂiss. ǂ24
Chronology:	6 Dec. 2022
DOI:	10.3390/plants11243468
ID:	26361726