Natural Deep Eutectic Solvent-Based Matrix Solid Phase dispersion (MSPD) extraction for determination of bioactive compounds from sandy everlasting (Helichrysum arenarium L.)

a case of stability study

Milena Ivanović (Avtor), Peter Krajnc (Avtor), Aleš Mlinarič (Avtor), Maša Islamčević Razboršek (Avtor)

Povzetek

In the present study, vortex-assisted matrix solid-phase dispersion (VA-MSPD) extraction was used to isolate the major bioactive compounds from H. arenarium. To reduce the negative environmental impact of the conventionally used organic solvents, four different choline chloride-based natural deep eutectic solvents (NADES) were investigated as possible eluents. The most influential VA-MSPD extraction parameters: stationary phase (adsorbent), adsorbent/sample ratio, vortex time, and volume of extraction solvent were systematically optimized. Ultrasound-assisted extraction with 80% MeOH was used as the standard method for the comparison of results. The stability of the obtained extracts was studied over a period of 0 to 60 days at three different temperatures (−18 ◦C, 4 ◦C, and 25 ◦C). All extracts were evaluated both spectrophotometrically (determination of total phenolic content (TPC) and antioxidant activity by ABTS and FRAP assay) and chromatographically (HPLC-UV). NADES based on choline chloride and lactic acid (ChCl-LA) was selected as the most effective extractant, with a determined TPC value of its extract of 38.34 ± 0.09 mg GA/g DW (27% higher than the methanolic VA-MSPD extract) and high antioxidant activity. The content of individual phenolic compounds (chlorogenic acid, dicaffeoylquinic acid isomers, naringenin isomers, and chalcones) in the ChCl-LA extract, determined by HPLC-UV, was comparable to that of the conventionally obtained one. Moreover, the stabilization effect of ChCl-LA was confirmed for the studied compounds: chlorogenic acid, naringenin-40 -O-glucoside, tomoroside A, naringenin-5-Oglucoside, isosalipurposide, and naringenin. The optimum VA-MSPD conditions for the extraction of H. arenarium polyphenols were: florisil/sample ratio of 0.5/1, a vortex time of 2 min, and an elution volume of ChCl-LA of 10 mL.

Ključne besede

fenoli;antioksidanti;ekstrakcija;matrix solid phase dispersion extraction;MSPD;natural deep eutectic solvents;NADES;extraction optimization;sandy everlasting;phenolic compounds;stability studies;antioxidant activity;

Podatki

Jezik:	Angleški jezik
Leto izida:	2022
Tipologija:	1.01 - Izvirni znanstveni članek
Organizacija:	UM FKKT - Fakulteta za kemijo in kemijsko tehnologijo
Založnik:	MDPI
UDK:	66.02
COBISS:	134436867
ISSN:	2223-7747
Št. ogledov:	0
Št. prenosov:	0
Ocena:	0 (0 glasov)
Metapodatki:

Ostali podatki

Sekundarni jezik:	Slovenski jezik
Sekundarne ključne besede:	fenoli;antioksidanti;ekstrakcija;
Vrsta dela (COBISS):	Članek v reviji
Strani:	17 str.
Letnik:	ǂVol. ǂ11
Zvezek:	ǂiss. ǂ24
Čas izdaje:	6 Dec. 2022
DOI:	10.3390/plants11243468
ID:	26361726