Secondary abstract: |
The goal of this thesis is to prove if the willow (Salix fragilis) extract influences the growth and development of Japanese knotweed (Fallopia japonica). Aquatic extract of 0,1 % and 1 % was gathered from the freeze-dried willow leaves. We have planted 27 Japanese knotweed sprouts, of approx. the same height. Ten of those were irrigated with the 0.1 % willow extract and 10 with the 1 % willow extract, while the remaining 5 with the distilled water (control group) and the 2 with the pipe water. The experiment lasted 196 days. For the first two months the plants were irrigated only once per month, from there on every 14 days. We have monitored the growth progress of shoot and the number of leaves on a weekly basis. At the end of the experiment, the masses of the plants' roots, stems and leaves were weighed and the biochemical measurements of the chosen stress parameters were carried out. Statistically significant differences in the growth of shoots and the number of leaves were not noticed. Even the photochemical efficiency was roughly the same, a slight increase of the potential photochemical efficiency was noticed in the control group. The average mass of the organs, especially the roots, was lower in the plants that were irrigated with the 1% water extract. Biochemical analyses have shown that with the plants that were irrigated with the water extract the protein content was lessened for about a quarter and, consequently, the enzyme activity was higher, especially in the roots. The measurements of the lipid peroxidation have revealed that the plants irrigated with the extract show more membrane damage in the leaves and roots, especially with the 1 % extract. Nonetheless, the results show that the water extract from willow caused stress in the plants, but the influence was not significant enough to affect any kind of physiological state of the exposed plants. |