Martin Fischlechner (Author), Markus Zaulig (Author), Stefan Meyer (Author), Irina Estrela-Lopis (Author), Luis Cuéllar (Author), Joseba Irigoyen (Author), Paula Pescador (Author), Milan Brumen (Author), Paul Messner (Author), Sergio Moya (Author), Edwin Donath (Author)

Abstract

The mechanism of formation of supported lipid layers from phosphatidylcholine and phosphatidylserine vesicles in solution on polyelectrolyte multilayers was studied by a variety of experimental techniques. The interaction of zwitterionic and acidic lipid vesicles, as well as their mixtures, with polyelectrolyte supports was followed in real time by micro-gravimetry. The fabricated lipid-polyelectrolyte composite structures on top of multilayer coated colloidal particles were characterized by flow cytometry and imaging techniques. Lipid diffusion over the macroscopic scale was quantified by fluorescence recovery after photobleaching, and the diffusion was related to layer connectivity. The phospholipid-polyelectrolyte binding mechanism was investigated by infrared spectroscopy. A strong interaction of polyelectrolyte primary amino groups with phosphate and carboxyl groups of the phospholipids, leading to dehydration, was observed. Long-range electrostatic attraction was proven to be essential for vesicle spreading and rupture. Fusion of lipid patches into a homogeneous bilayer required lateral mobility of the lipids on the polyelectrolyte support. The binding of amino groups to the phosphate group of the zwitterionic lipids was too weak to induce vesicle spreading, but sufficient for strong adsorption. Only the mixture of phosphatidylcholine and phosphatidylserine resulted in the spontaneous formation of bilayers on polyelectrolyte multilayers. The adsorption of phospholipids onto multilayers displaying quarternary ammonium polymers produced a novel 3D lipid polyelectrolyte structure on colloidal particles.

Keywords

biofizika;lipidi;lipidne plasti;elektroliti;biophysics;lipids;lipid layers;electrolytes;multilayer supports;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UM FNM - Faculty of Natural Sciences and Mathematics
UDC: 577.115:53.082.75
COBISS: 16347912 Link will open in a new window
ISSN: 1744-683X
Views: 1109
Downloads: 58
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Other data

Secondary language: English
Secondary keywords: biofizika;lipidi;lipidne plasti;elektroliti;
URN: URN:SI:UM:
Pages: str. 2245-2258
Volume: 4
Issue: ǂiss. ǂ11
Chronology: 2008
DOI: 10.1039/b805754k
ID: 8724044