Uta Reibetanz (Avtor), David Haložan (Avtor), Milan Brumen (Avtor), Edwin Donath (Avtor)

Povzetek

Polyelectrolyte multilayer sensor capsules, 5 m in diameter, which contained fluorescein-labeled poly(acrylic acid) (PAAAF) as pH-sensitive reporter molecules, were fabricated and employed to explore their endocytotic uptake into HEK 293T cells by flow cytometry. The percentage of capsules residing in the endolysosomal compartment was estimated from the fluorescence intensity decrease caused by acidification. Capsules attached to the extracellular surface of the plasma membrane were identified by trypan blue quenching. The number of capsules in the cytoplasm was rather small, being below the detection limit of the method. The advantages of polyelectrolyte multilayer capsules are that the fluorophore is protected from interaction with cellular compartments and that the multilayer can be equipped with additional functions.

Ključne besede

biofizika;polielektroliti;večplastne kapsule;polielektrolitne kapsule;biophysics;polyelectrolytes;multilayer capsules;polyelectrolyte capsules;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UM FNM - Fakulteta za naravoslovje in matematiko
UDK: 577.3
COBISS: 15383304 Povezava se bo odprla v novem oknu
ISSN: 1525-7797
Št. ogledov: 1122
Št. prenosov: 101
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Angleški jezik
Sekundarne ključne besede: biofizika;polielektroliti;večplastne kapsule;polielektrolitne kapsule;
URN: URN:SI:UM:
Strani: str. 1927-1933
Letnik: ǂVol. ǂ8
Zvezek: ǂiss. ǂ6
Čas izdaje: 2007
ID: 8724117