Flow cytometry of HEK 293T cells interacting with polyelectrolyte multilayer capsules containing fluorescein-labeled poly(acrylic acid) as a pH sensor

Uta Reibetanz (Author), David Haložan (Author), Milan Brumen (Author), Edwin Donath (Author)

Abstract

Polyelectrolyte multilayer sensor capsules, 5 m in diameter, which contained fluorescein-labeled poly(acrylic acid) (PAAAF) as pH-sensitive reporter molecules, were fabricated and employed to explore their endocytotic uptake into HEK 293T cells by flow cytometry. The percentage of capsules residing in the endolysosomal compartment was estimated from the fluorescence intensity decrease caused by acidification. Capsules attached to the extracellular surface of the plasma membrane were identified by trypan blue quenching. The number of capsules in the cytoplasm was rather small, being below the detection limit of the method. The advantages of polyelectrolyte multilayer capsules are that the fluorophore is protected from interaction with cellular compartments and that the multilayer can be equipped with additional functions.

Keywords

biofizika;polielektroliti;večplastne kapsule;polielektrolitne kapsule;biophysics;polyelectrolytes;multilayer capsules;polyelectrolyte capsules;

Data

Language:	English
Year of publishing:	2007
Typology:	1.01 - Original Scientific Article
Organization:	UM FNM - Faculty of Natural Sciences and Mathematics
UDC:	577.3
COBISS:	15383304
ISSN:	1525-7797
Views:	1122
Downloads:	101
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary keywords:	biofizika;polielektroliti;večplastne kapsule;polielektrolitne kapsule;
URN:	URN:SI:UM:
Pages:	str. 1927-1933
Volume:	ǂVol. ǂ8
Issue:	ǂiss. ǂ6
Chronology:	2007
ID:	8724117