magistrsko delo
Marko Munda (Author), Lidija Fras Zemljič (Mentor), Ivan Kosalec (Co-mentor), Simona Strnad (Co-mentor)

Abstract

V nalogi bosta z vidika protimikrobnega učinka raziskana hitozan v raztopini, kot tudi celuloza funkcionalizirana s hitozanom. Hitozan poseduje protimikrobne lastnosti, kar pa zavisi od številnih dejavnikov, kot recimo števila protoniranih aminskih skupin, molske mase, topila, itd... Običajno protimikrobna sredstva preprečujejo celično delitev bakterij, poškodujejo celično steno ali vplivajo na prepustnost celične membrane, denaturirajo proteine, blokirajo aktivnost encimov in tako onemogočajo preživetje celic. Pri inhibiciji patogenih mikroorganizmov se sprošča intracelularni kationski kalij, njegova kvantitativna določitev pa omogoča spremljanje baktericidnega učinka protimikrobnih sredstev. Slednje je mogoče izkoristiti kot alternativno tehniko za določitev inhibicije mikroorganizmov v stiku s hitozanom oz. protimikrobno funkcionalizirano celulozo. Ker na področju testiranja vlaken obstaja dokaj velika vrzel je uspešna vzpostavitev konvencionalnih tehnik izredno dobrodošla. Cilj magistrske naloge je tako uporaba atomske emisijske spektrofotometrije in potenciometričnih titracij z kalijevo ion selektivno elektrodo za kvantitativno analizo intracelularnega kationskega kalija, nastalega pri degradaciji mikroorganizmov v stiku s protimikrobnimi materiali. Eden izmed pomembnejših ciljev je natančna pridobitev supernatanta z vsebnostjo intracelularnega kationskega kalija brez prisotnosti mikroorganizmov, za kar bo potrebno po interakciji specifičnega mikroorganizma z raztopino hitozana in celulozo funkcionalizirano z raztopino hitozana izvesti ultrafiltracijo. Slednje omogoča varno uporabo omenjenih tehnik za določitev protimikrobnega učinka uporabljenih materialov. Podana bo primerjava med protimikrobno učinkovitostjo (zaradi sproščenega kalija) raztopine hitozana in funkcionalizirano površino celuloze s hitozanom.

Keywords

celuloza;hitozan;Candida albicans;atomska emisijska spektroskopija;potenciometrične titracije s kalijevo ion-selektivno elektrodo;magistrske naloge;

Data

Language: Slovenian
Year of publishing:
Typology: 2.09 - Master's Thesis
Organization: UM FS - Faculty of Mechanical Engineering
Publisher: [M. Munda]
UDC: 661.728.7:677.016(043.2)
COBISS: 17852950 Link will open in a new window
Views: 1276
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Other data

Secondary language: English
Secondary title: Determination of antimicrobical efficiency of medical textiles
Secondary abstract: antimicrobial characteristics, which depend on number of factors, such as the number of protonated amine groups, molecular weight, solvents, etc. Antimicrobials prevent bacterial cell division by damaging the cell wall or affecting the permeability of cell membranes; they denature proteins, block enzyme activity, preventing cell survival. In the inhibition of pathogenic microorganisms, intracellular potassium cations are released. Their quantitative determination enables monitoring of the bactericidal effect of antimicrobials. It can be used as an alternative technique for determining the inhibition of microorganisms in contact with chitosan or antimicrobial functionalized cellulose. Since there is a large gap in the field of fibre testing, the successful establishment of standard techniques is extremely welcome. Thus, the intention of the Master's Thesis is the use of atomic emission spectrophotometry and potentiometric titration with a potassium ion-selective electrode for the quantitative analysis of intracellular potassium cations, resulting from the degradation of microorganisms in contact with antimicrobial materials. One of the major objectives is to obtain an accurate supernatant that contains expelled intracellular potassium cations without the presence of microorganisms, which requires the implementation of ultrafiltration after the interaction of specific microorganism with a solution of chitosan and cellulose functionalized with a solution of chitosan. This allows safe use of these techniques for determination of antimicrobial effect of the materials used. A comparison between the antimicrobial efficacy (due to released potassium) of a solution of chitosan, and a cellulose surface functionalized with chitosan, will be presented.
Secondary keywords: cellulose;chitosan;Candida albicans;atomic emission spectroscopy;potentiometric titration with potassium ion-selective electrode;
URN: URN:SI:UM:
Type (COBISS): Master's thesis/paper
Thesis comment: Univ. v Mariboru, Fak. za strojništvo
Pages: IX, 47 f.
ID: 8729239