Vpliv inkapsulacijske dehidracije na morfologijo, regeneracijo in aklimatizacijo in vitro vzgojene rebrinčevolistne hladnikovke (Hladnikia pastinacifolia Rchb.)

diplomsko delo

Marijana Baraba (Author), Jana Ambrožič-Dolinšek (Mentor), Terezija Ciringer (Co-mentor)

Abstract

Rebrinčevolistna hladnikovka ali hladnikija (Hladnikia plastinacifolia Rchb.) je ledenodobna endemična vrsta, ki v naravi raste samo na majhnem območju Trnovskega gozda v Sloveniji in je zato zelo ranljiva vrsta. Zavarovana je in situ in ex situ. Ex situ jo ohranjajo v botaničnem vrtu v Ljubljani. In situ jo hranimo in vitro na Fakulteti za naravoslovje in matematiko Univerze v Mariboru, kjer razvijamo postopke za dolgoročno hrambo s pomočjo krioprezervacije z inkapsulacijsko dehidracijo (ID). Krioprezervacijske metode lahko vplivajo na gensko stabilnost rastlinskega materiala, te pa na spremenjeno morfologijo rastline. Namen diplomske naloge je raziskati, ali krioprezervacija z ID izzove morfološke spremembe rastlinskega materiala. Primerjali smo morfološke značilnosti treh nekrioprezerviranih (ne-krio) in krioperezerviranih (krio) linij hladnikovke. Med vcepki ne-krio in krio linij večinoma nismo opazili statistično značilnih razlik v številu novih brstov, višini vcepkov, prisotnosti kalusa ter številu in dolžini korenin. Ne-krio vcepki so regenerirali v povprečju 1,2 brsta na vcepek. Vcepki krio linij so imeli v povprečju 1,8 brsta na vcepek, a so bili ti v povprečju nekoliko nižji kot vcepki krio linij. Pri ne-krio vcepkih se je tvorilo več kalusa kot pri krio vcepkih. Korenine so se tvorile na več kot 90 % ne-krio vcepkov, ki so imeli najpogosteje več kot 15 korenin. Na krio vcepkih so se korenine tvorile na 77,6 % vcepkov, in sicer so se najpogosteje tvorile od 1 do 4 korenine. Zadnja faza mikropropagacije, aklimatizacija vcepkov, je trajala 75 dni v rastni komori in nato 25 dni na prostem, v gredi so preživeli samo trije krio vcepki. V prihodnje bo treba optimizirati proces aklimatizacije.

Keywords

diplomska dela;rebrinčevolistna hladnikovka;tkivna kultura;mikropropagacija;morfološke značilnosti rastlin;aklimatizacija;krioprezervacija;inkapsulacijska dehidracija;

Data

Language:	Slovenian
Year of publishing:	2015
Typology:	2.11 - Undergraduate Thesis
Organization:	UM FNM - Faculty of Natural Sciences and Mathematics
Publisher:	[M. Baraba]
UDC:	582.795:581.4(043.2)
COBISS:	21647368
Views:	1560
Downloads:	87
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	The influence of encapsulation dehydration on morphology, regeneration and acclimatisation of in vitro established Hladnikia pastinacifolia Rchb.
Secondary abstract:	Maribor takes care of its in situ storage. It keeps Hladnikia plastinacifolia in vitro and develops protocols for its long-term storage by cryopreservation with encapsulation dehydration (ID). Cryopreservation methods can cause changes in genetic stability of used material which can be expressed in changed morphology.The aim of the thesis is to research if cryopreservation with IDcauses morphological changes on plant material. We compared morphological characteristics of three cryopreserved (krio) and control lines that were not cryopreserved (ne-krio) of Hladnikia plastinacifolia. There were no essential differences in the number and height of the new formed buds and in callus formation nor in the number and length of roots. Control lines had 1.2 buds in average. Cryopreserved lines had 1.8 buds in average, but they were a bit shorter in average than those in control lines. Callus formed more often on control buds. The roots formed on more than 90 % of control samples and most often their numerous preceded 15 roots per sample. Cryopreserved buds formed formed roots only on 77.6 % of all samples. The last phase of micropropagation, acclimatisation of plantlets, lasted 75 days in a growing chamber and afterwards for 25 days outside, in the strand survived only three kriopresrved plantlets. For the higher survival rate the optimization of the acclimatization process needs to be improved.
Secondary keywords:	theses;Hladnikia plastinacifolia Rchb.;tissue culture;micropropagation;acclimatization;cryopreservation;cryopreserved and unpreserved lines;
URN:	URN:SI:UM:
Type (COBISS):	Undergraduate thesis
Thesis comment:	Univ. v Mariboru, Fak. za naravoslovje in matematiko, Oddelek za biologijo
Pages:	VIII, 48 f.
ID:	8889445