Formulacija biološko aktivnih učinkovin s superkritičnimi fluidi

magistrsko delo

Klara Bigec (Author), Željko Knez (Mentor), Maša Knez Hrnčič (Co-mentor)

Abstract

V sklopu magistrske naloge z naslovom Formulacija biološko aktivnih učinkovin s superkritičnimi fluidi smo uporabili različne postopke za mikronizacijo. S pomočjo termogravimetrične analize/ diferencialne dinamične kalorimetrije (TGA/DSC) smo preučevali vpliv tlaka in atmosfere na vzorec, kjer je pod visokim tlakom zanemarjen vpliv izhlapevanja, adsorpcije in desorpcije, vpliv tlaka na temperaturo vrelišča oz. tališča ter določili pristnost učinkovine, glede na podatke najdene v literaturi. Določevali smo topnost izbrane učinkovine v CO2, propanu in argonu v visokotlačni optični celici pri sub- in superkritičnih pogojih (pri treh različnih temperaturah, 40° C, 60° C in 80° C ter tlakih do 450 bar). Hkrati smo učinkovini določili tališče pod tlakom sub- in superkritičnega CO2, propana ter argona v odvisnosti od tlaka in ob počasnem zviševanju temperature. Vzorce, katerim smo določali tališča, smo analizirali s pomočjo tekočinske kromatografije z masno spektrometrijo (LC-MS). Uporabili smo tudi eno izmed visokotlačnih metod za zmanjševanje velikosti delcev s superkritičnimi fluidi PGSSTM (Particles from Gas Saturated Solutions = delci iz raztopin, nasičenih s plinom). Kot vzorčni sistem smo uporabili polimer PEG 6000/superkritični CO2/aktivna komponenta. Ta proces uporabljamo pri mikronizaciji za pridobivanje delcev velikostnega reda mikrometer (µm), ki absorbirajo velike koncentracije superkritičnih fluidov. [1] Dobljene delce smo analizirali z LC-MS in TGA/DSC.

Keywords

esomeprazol;superkritični fluidi;topnost;farmacevtske učinkovine;mikronizacija;magistrske naloge;

Data

Language:	Slovenian
Year of publishing:	2016
Typology:	2.09 - Master's Thesis
Organization:	UM FKKT - Faculty of Chemistry and Chemical Engineering
Publisher:	[K. Bigec]
UDC:	54-139:543.544.5(043.2)
COBISS:	20356630
Views:	1349
Downloads:	163
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	Formulation of bioactive substances with supercritical fluids
Secondary abstract:	Within the master degree entitled “Formulation of bioactive substances with supercritical fluids”, we used different procedures for micronization. By using thermogravimetric analysis/ differential dynamic calorimetry (TGA/DSC) the impact of pressure and atmosphere on a sample, where impact of evaporation, adsorption and desorption under the high pressure is neglected, the impact of pressure on the temperature of a melting or boiling point and to identify the pharmaceutical active ingredient, according to the data found in the literature were studied. Solubility of esomprazole as an active compound was investigated in CO2, propane and argon in a high pressure view cell at three different temperatures of 40° C, 60° C and 80° and at pressures up to 450 bars. Melting points of active compound were determined in sub- and supercritical CO2, propane and argon as a function of pressure while gradually raising temperature in a high pressure view cell. Samples, which were subjected to determination of melting point were later analysed by using a liquid chromatography coupled with mass spectometry (LC-MS). PGSSTM technique was applied for the production of polymer powder and for the entrapping of active ingredient in polymer matrice as a modern concept for reducing the particle size with supercritical fluids. Polymer PEG 6000/supercritical CO2/active component system was used as model systems. This process is used for micronization to obtain particles of the order of a micrometer (µm), which absorb high concentrations of supercritical fluids. [1] Obtained particles were further analyzed with LC-MS and TGA/DSC.
Secondary keywords:	esomeprazole;supercritical fluids;solubility;pharmaceutical compound;micronization;
URN:	URN:SI:UM:
Type (COBISS):	Master's thesis/paper
Thesis comment:	Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Pages:	IX, 49 f.
ID:	9166803