Elisa Avolio (Author), Giuseppe Gianfranceschi (Author), Daniela Cesselli (Author), Angela Caragnano (Author), Emmanouil Athanasakis (Author), Rajesh Katare (Author), Marco Meloni (Author), Anita Palma (Author), Arianna Barchiesi (Author), Carlo Vascotto (Author), Elisa Mazzega (Author)

Abstract

Cardiac stem cells (CSC) from explanted decompensated hearts (E-CSC) are, with respect to those obtained from healthy donors (D-CSC), senescent and functionally impaired. We aimed to identify alterations in signaling pathways that are associated with CSC senescence. Additionally, we inves- tigated if pharmacological modulation of altered pathways can reduce CSC senescence in vitro and enhance their reparative ability in vivo. Measurement of secreted factors showed that E-CSC release larger amounts of proinflammatory cytokine IL1b compared with D-CSC. Using blocking antibodies, we verified that IL1b hampers the paracrine protective action of E-CSC on cardiomyo- cyte viability. IL1b acts intracranially inducing IKKb signaling, a mechanism that via nuclear factor-jB upregulates the expression of IL1b itself. Moreover, E-CSC show reduced levels of AMP protein kinase (AMPK) activating phosphorylation. This latter event, together with enhanced IKKb signaling, increases TORC1 activity, thereby impairing the autophagic flux and inhibiting the phos- phorylation of Akt and cAMP response element-binding protein. The combined use of rapamycin and resveratrol enhanced AMPK, thereby restoring downstream signaling and reducing IL1b secretion. These molecular corrections reduced E-CSC senescence, re-establishing their protective activity on cardiomyocytes. Moreover ex vivo treatment with rapamycin and resveratrol improved E-CSC capacity to induce cardiac repair upon injection in the mouse infarcted heart, leading to reduced cardiomyocyte senescence and apoptosis and increased abundance of endog- enous c-Kit1 CSC in the peri-infarct area. Molecular rejuvenation of patient-derived CSC by short pharmacologic conditioning boosts their in vivo reparative abilities. This approach might prove useful for refinement of CSC-based therapies.

Keywords

stem cells;myocardial infarction;heart failure;cellular senescence;

Data

Language: English
Year of publishing:
Typology: 1.01 - Original Scientific Article
Organization: UNG - University of Nova Gorica
UDC: 577
COBISS: 4723963 Link will open in a new window
ISSN: 1066-5099
Views: 3382
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Other data

URN: URN:SI:UNG
Type (COBISS): Not categorized
Pages: str. 2373-2385
Volume: ǂVol. ǂ32
Issue: ǂiss. ǂ9
Chronology: 2014
DOI: 10.1002/stem.1728
ID: 9593049