Vpliv superkritičnega ogljikovega dioksida na izločanje proteinov in nukleinskih kislin iz glive Hortae werneckii

diplomsko delo

Diana Smej (Author), Maja Leitgeb (Mentor)

Abstract

Hortaea werneckii je zaradi izjemne tolerantnosti na slana območja, postala evkariontski model organizma za prilagoditev na slanost. Različne študije so dokazale, da ima ta gliva več mehanizmov za prilagoditev na zelo slana območja. Superkritični ogljikov dioksid se že nekaj časa uporablja v medicini in prehrambni industriji za sterilizacijo materialov in za zmanjševanje aktivnosti nekaterih organizmov, kot so bakterije in glive. Pri visokih tlakih lahko povzroči celično smrt, pri nižjih tlakih pa poškoduje celice do te mere, da se intracelične spojine in strukture izločijo iz celice. Cilj diplomskega dela je določiti preživelost glive H. werneckii po izpostavljanju v SC CO2, ugotoviti, kako le-ta vpliva na aktivnost izločenih encimov in koliko proteinov in nukleinskih kislin se bo izločilo po tem, ko smo glivo izpostavili v SC CO2 pri tlaku 100 in 300 bar in različnih časovnih obdobjih.

Keywords

diplomska dela;Hortae werneckii;glive;proteini;nukleinske kisline;superkritični ogljikov dioksid;

Data

Language:	Slovenian
Year of publishing:	2012
Source:	Maribor
Typology:	2.11 - Undergraduate Thesis
Organization:	UM FNM - Faculty of Natural Sciences and Mathematics
Publisher:	[D. Smej]
UDC:	54(043.2)
COBISS:	19591432
Views:	1863
Downloads:	106
Average score:	0 (0 votes)
Metadata:

Other data

Secondary language:	English
Secondary title:	INFLUENCE OF SUPERCRITICAL CARBON DIOXIDE ON THE SECRETION OF PROTEINS AND NUCLEIC ACID FROM FUNGI HORTAEA WERNECKII
Secondary abstract:	Hortaea werneckii is due to the exceptional tolerance to saltwater areas become eukaryotic model organism for adaptation to salinity. Various studies have demonstrated that this fungus has several mechanisms to adapt to highly saline areas. Supercritical carbon dioxide is already used in medicine and food industry to sterilize materials and to reduce the activities of some organisms, such as bacteria and fungi. At high pressures can cause cell death and at lower pressures damaged cells to such an extent, that the intracellular compounds and the structure extracted from the cell. The purpose of diploma work paper was to determine the survival of fungus H. werneckii after exposure in SC CO2, determine how it will affect on activity of eliminated enzymes and how many proteins and nucleic acids will be excreted after we are expose H. werneckii in SC CO2 at a pressure of 100 and 300 bar and different time periods.
Secondary keywords:	Hortaea werneckii;supercritical carbon dioxide;activity of enzymes;proteins;nucleic acids;
URN:	URN:SI:UM:
Type (COBISS):	Undergraduate thesis
Thesis comment:	Univ. v Mariboru, Fak. za naravoslovje in matematiko, Oddelek za kemijo
Pages:	57 f.
Keywords (UDC):	mathematics;natural sciences;naravoslovne vede;matematika;chemistry;crystallography;mineralogy;kemija;
ID:	999793