magistrsko delo
Povzetek
Mikro RNA so kratke nekodirajoče RNA, ki uravnavajo izražanje genov. Preko vplivov na razne biološke poti v organizmu lahko prispevajo tudi k spremembi fenotipa in posledično k uspešnosti prireje rejnih živali. S tem postanejo miRNA uporabne tudi kot biooznačevalci v selekciji živali. Za analizo smo izbrali miRNA gen gga-mir-1658 pri kokoši. Namen te naloge je bil: 1. pridobiti znane genomske podatke o gga-mir-1658 pri kokoši in 2. z molekularno-genetskimi metodami analizirati povezavo med SNP-ji (rs316300182 in rs1059378319) v gga-mir-1658 ter desetimi pitovnimi in klavnimi lastnostmi kokoši. V prvem delu smo informacije o gga-mir-1658 iz osmih podatkovnih zbirk in bioinformacijskih orodij združili v atlas regulatornih elementov. Z asociacijsko analizo smo nato potrdili povezavo med SNP-jem rs1059378319 in maso jeter (p = 0,05). Živali z genotipom CC so imele za 5,91 g težja jetra (p = 0,02) kot živali z genotipom CT. Povezava med rs316300182 in maso jeter je bila le nakazana (p = 0,07). Živali z genotipom CC so imele za 5,66 g težja jetra (p = 0,06) kot živali z genotipom CG. Povezave ostalih izmerjenih lastnosti s prej omenjenima SNP-jema niso bile značilne. Rezultati nakazujejo na to, da je gga-mir-1658 zanimiva za nadaljnje raziskave. S testiranjem na večji in pasemsko bolj raznoliki populaciji bo možno bolje raziskati njen funkcionalni vpliv in njen potencial biooznačevalca v selekciji kokoši.
Ključne besede
perutnina;kokoši;pitovni piščanci;pitovne lastnosti;klavne lastnosti;genetika;nekodirajoče RNA;genetska variabilnost;magistrske naloge;
Podatki
Jezik: |
Slovenski jezik |
Leto izida: |
2018 |
Tipologija: |
2.09 - Magistrsko delo |
Organizacija: |
UL BF - Biotehniška fakulteta |
Založnik: |
[N. Pogorevc] |
UDK: |
636.5: 575(043.2) |
COBISS: |
4126088
|
Št. ogledov: |
862 |
Št. prenosov: |
261 |
Ocena: |
0 (0 glasov) |
Metapodatki: |
|
Ostali podatki
Sekundarni jezik: |
Angleški jezik |
Sekundarni naslov: |
Association analysis between genetic variability of selected non-coding RNAs with growth and carcass traits in chicken |
Sekundarni povzetek: |
MicroRNAs are small noncoding RNAs that regulate gene expression. Through effects on various biological pathways in organism, miRNAs contribute to phenotype changes and consequently on efficiency of animal production. That is why miRNAs became useful as biomarkers in animal selection. For the analysis we selected miRNA gene gga-miR-1658. The aim of this study was: 1.) to retrieve known genomic information about gga-mir-1658 in chicken and 2.) to analyze the association between two SNPs (rs316300182 and rs1059378319) in gga-mir-1658 gene and ten growth and carcass traits using molecular-genetic methods. Firstly, we designed an atlas of regulatory elements for gga-mir-1658 from eight databases and bioinformatics tools. In the second part, we confirmed an association between rs1059378319 and liver mass (p = 0.05). Animals with CC genotype had 5.91g heavier liver (p = 0.02) than animals with CT genotype. Association between SNP rs316300182 and liver mass (p = 0.07) did not meet the level of significance, but was close. Animals with CC genotype had for 5.66g heavier liver (p = 0.06) as animals with a genotype CG. Other growth and carcass traits did not reach significant associations with these two SNPs. Results suggest that gga-mir-1658 is interesting for further studies. Testing on a larger population and including additional breeds is required to functionally characterize it and potentially apply it as a biomarker in chicken selection programs. |
Sekundarne ključne besede: |
poultry;chicken;broilers;growth;carcass traits;genetics;non-coding RNA;genetic variability; |
Vrsta dela (COBISS): |
Magistrsko delo/naloga |
Študijski program: |
0 |
Konec prepovedi (OpenAIRE): |
1970-01-01 |
Komentar na gradivo: |
Univ. v Ljubljani, Biotehniška fak., Oddelek za zootehniko |
Strani: |
IX, 29 str., 2 str. pril. |
ID: |
10959867 |