Interakcije biotehnološko relevantnih encimov

magistrsko delo

Barbara Grabrovec (Avtor), Urban Bren (Mentor), Maja Leitgeb (Komentor), Janez Konc (Komentor)

Povzetek

Encimi so protein, ki pospešujejo biokemijske in kemijske reakcije. Njihove interakcije z ligandi so ključnega pomena za številne biokemične interakcije, ki uravnavajo življenje. Uporabljajo se v biotehnologiji, pri analitskih procesih, v človeški in živalski terapiji (za zdravila) ter v različnih industrijskih procesih, kamor spadajo tekstilna industrija, usnjarska industrija, farmacija, živilska industrija in druge. Kljub vsem dobrim lastnostim encimov, njihovo široko industrijsko uporabo pogosto ovirajo kratkotrajna stabilnost, rok uporabe ter njihova nepopolna regeneracija in ponovna uporaba. Te pomanjkljivosti lahko na splošno odpravimo z imobilizacijo encimov. V zadnjih letih so se pojavile podatkovne baze, ki vsebujejo podatke o interakcijah protein-ligand, kar je pripomoglo k razumevanju funkcij novo odkritih proteinov. Razvile so se številne nove računalniške metode, ki lahko uporabijo informacije shranjenih interakcij, da napovedo nove neznane interakcije. Najpogosteje uporabljena metoda za modeliranje interakcij je metoda molekulskega sidranja, ki se uporablja za simuliranje interakcije med majhno molekulo in proteinom na atomski ravni in nam opiše obnašanje majhne molekule v vezavnem mestu encima. V magistrskem delu smo s pomočjo programa molekulskega sidranja na encime vezali nizkomolekularne ligande. Strukture encimov smo pridobili v spletni bazi PDB, datoteke ligandov pa v spletni podatkovni bazi ZINC15. Kot rezultat smo dobili strukture ligandov, ki so se najmočneje vezali in njihovo energijo vezave. Rezultate smo pregledali s programoma Excel in Pymol, kjer smo videli 3D slike ligandov, ki so se na naše encime vezali z najnižjo energijo.

Ključne besede

encim;ligand;imobilizacija;biotehnologija;molekularno modeliranje;molekulsko sidranje;magistrske naloge;

Podatki

Jezik:	Slovenski jezik
Leto izida:	2019
Tipologija:	2.09 - Magistrsko delo
Organizacija:	UM FKKT - Fakulteta za kemijo in kemijsko tehnologijo
Založnik:	[B. Grabrovec]
UDK:	577.15:544.139(043.2)
COBISS:	22689046
Št. ogledov:	777
Št. prenosov:	60
Ocena:	0 (0 glasov)
Metapodatki:

Ostali podatki

Sekundarni jezik:	Angleški jezik
Sekundarni naslov:	Interactions of biotechnologically relevant enzymes
Sekundarni povzetek:	Enzymes are proteins, that accelerate biochemical and chemical reactions. Their interactions with ligands are crucial for many life-regulating biochemical interactions. They are used in biotehnology, in analytical processes, in human and animal therapy (as medicines) and in various industrial processes such as textile, leather, pharmacy, food and others. Despite of all the good properties of enzymes, their widespread industrial use is often hampered by long-term stability, shelf life and their cumbersome renewal and reuse. These disadvantages can generally be eliminated using enzyme immobilization. In recent years, databases containing protein-ligand interactions data have emerged, which has helped us to understand the functions of newly discovered proteins. Many new computional methods have been developed that can use the stored interaction information to predict new uknown interactions. The most commonly used interaction modeling method is molecular docking, which is used to model interactions between a small molecule and a protein on an atomistic scale, as well as to describe the behaviour of small molecules in enzyme binding sites. In this master's thesis low molecular ligands were attached to enzymes using computional method called molecular docking. Enzyme structures were obtained from PDB online database and ligand files were obtained from the ZINC15 online database. As a result we obtained the structures of ligands that bonded best as well as their binding energy. We reviewed the results using Excel and Pymol, where we got 3D structures of ligands that attached to our enzymes with the lowest energy.
Sekundarne ključne besede:	enzyme;ligand;biotehnology;molecular modeling;molecular docking;
Vrsta dela (COBISS):	Magistrsko delo/naloga
Komentar na gradivo:	Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Strani:	VIII, 42 str.
ID:	11232395