Branislava Ranković (Avtor), Nina Zidar (Avtor), Margareta Žlajpah (Avtor), Emanuela Boštjančič (Avtor)

Povzetek

MicroRNAs of the miR-200 family have been shown experimentally to regulate epithelialmesenchymal transition (EMT). Although EMT is the postulated mechanism of development and progression of colorectal cancer (CRC), there are still limited and controversial data on expression of miR-200 family and their target genes during CRC cancerogenesis. Our study included formalinfixed paraffin-embedded biopsy samples of 40 patients (10 adenomas and 30 cases of CRC with corresponding normal mucosa). Expression of miR-141, miR-200a/b/c and miR-429 and their target genes (CDKN1B, ONECUT2, PTPN13, RND3, SOX2, TGFB2 and ZEB2) was analysed using quantitative real-time PCR. Expression of E-cadherin was analysed using immunohistochemistry. All miRNAs were down-regulated and their target genes showed the opposite expression in CRC compared to adenoma. Down-regulation of the miR-200 family at the invasive front in comparison to the central part of tumour was observed as well as a correlation of expression of miR-200b, CDKN1B, ONECUT2 and ZEB2 expression to nodal metastases. Expression of the miR-200 family and SOX2 also correlated with E-cadherin staining. These results suggest that the miR-200 family and their target genes contribute to progression of adenoma to CRC, invasive properties and development of metastases. Our results strongly support the postulated hypotheses of partial EMT and intra-tumour heterogeneity during CRC cancerogenesis.

Ključne besede

colorectal adenoma;colorectal carcinoma;metastases;intra-tumour heterogeneity;epithelial-mesenchymal transition;miR-200 family;target genes;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL MF - Medicinska fakulteta
UDK: 616-006
COBISS: 34547929 Povezava se bo odprla v novem oknu
ISSN: 2077-0383
Št. ogledov: 322
Št. prenosov: 81
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: kolorektalni adenom;kolorektalni karcinom;metastaze;intra-tumorska heterogenost;epitelno-mezenhimska tranzicija;družina miR-200;tarčni geni;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-22
Letnik: ǂVol. ǂ8
Zvezek: ǂiss. ǂ10
Čas izdaje: 2019
DOI: 10.3390/jcm8101603
ID: 13188525