Ana Pavlič (Avtor), Emanuela Boštjančič (Avtor), Rajko Kavalar (Avtor), Bojan Ilijevec (Avtor), Serena Bonin (Avtor), Fabrizio Zanconati (Avtor), Nina Zidar (Avtor)

Povzetek

Morphological features including infiltrative growth, tumour budding (TB), and poorly differentiated clusters (PDCs) have a firmly established negative predictive value in colorectal cancer (CRC). Despite extensive research, the mechanisms underlying different tumour growth patterns remain poorly understood. The aim of this study was to investigate the involvement of epithelial-mesenchymal transition (EMT) in TB and PDCs in CRC. Using laser-capture microdissection, we obtained distinct parts of the primary CRC including TB, PDCs, expansive tumour front, and the central part of the tumour, and analysed the expression of EMT-related markers, i.e. the miR-200 family, ZEB1/2, RND3, and CDH1. In TB, the miR-200 family and CDH1 were significantly downregulated, while ZEB2 was significantly upregulated. In PDCs, miR-141, miR-200c, and CDH1 were significantly downregulated. No significant differences were observed in the expression of any EMT-related markers between the expansive tumour front and the central part of the tumour. Our results suggest that both TB and PDCs are related to partial EMT. Discrete differences in morphology and expression of EMT-related markers between TB and PDCs indicate that they represent different manifestations of partial EMT. TB seems to be closer to complete EMT than PDCs.

Ključne besede

colon cancer;colorectal cancer;epithelial-mesenchymal transition;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL MF - Medicinska fakulteta
UDK: 616
COBISS: 121432323 Povezava se bo odprla v novem oknu
ISSN: 0022-3417
Št. ogledov: 54
Št. prenosov: 31
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: rak debelega črevesa;kolorektalni rak;epitelno-mezenhimski prehod;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 278-288
Letnik: ǂVol. ǂ258
Zvezek: ǂno. ǂ3
Čas izdaje: Nov. 2022
DOI: 10.1002/path.5998
ID: 16736381
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