Povzetek
Staphylococcus epidermidis cleanroom strains are often exposed to sub-inhibitory concentrations of disinfectants, including didecyldimethylammonium chloride (DDAC). Consequently, they can adapt or even become tolerant to them. RNA-sequencing was used to investigate adaptation and tolerance mechanisms of S. epidermidis cleanroom strains (SE11, SE18), with S. epidermidis SE11Ad adapted and S. epidermidis SE18To tolerant to DDAC. Adaptation to DDAC was identified with up-regulation of genes mainly involved in transport (thioredoxin reductase [pstS], the arsenic efflux pump [gene ID, SE0334], sugar phosphate antiporter [uhpT]), while down-regulation was seen for the Agr system (agrA, arC, agrD, psm, SE1543), for enhanced biofilm formation. Tolerance to DDAC revealed the up-regulation of genes associated with transporters (L-cysteine transport [tcyB]; uracil permease [SE0875]; multidrug transporter [lmrP]; arsenic efflux pump [arsB]); the down-regulation of genes involved in amino-acid biosynthesis (lysine [dapE]; histidine [hisA]; methionine [metC]), and an enzyme involved in peptidoglycan, and therefore cell wall modifications (alanine racemase [SE1079]). We show for the first time the differentially expressed genes in DDAC-adapted and DDAC-tolerant S. epidermidis strains, which highlight the complexity of the responses through the involvement of different mechanisms.
Ključne besede
mikrobiološka kontrola obratov;čisti prostori;patogeni mikroorganizmi;Staphylococcus epidermidis;razkuževanje;didecildimetilamonijev klorid;DDAC;sekvencioniranje RNA;RNA -seq;biofilmi;izlivne črpalke;mehanizmi prilagoditve;
Podatki
Jezik: |
Angleški jezik |
Leto izida: |
2020 |
Tipologija: |
1.01 - Izvirni znanstveni članek |
Organizacija: |
UL BF - Biotehniška fakulteta |
UDK: |
579.24+579.26:613.6 |
COBISS: |
5173368
|
ISSN: |
2076-2607 |
Št. ogledov: |
143 |
Št. prenosov: |
64 |
Ocena: |
0 (0 glasov) |
Metapodatki: |
|
Ostali podatki
Sekundarni jezik: |
Slovenski jezik |
Sekundarne ključne besede: |
mikrobiološka kontrola obratov;čisti prostori;patogeni mikroorganizmi;Staphylococcus epidermidis;razkuževanje;didecildimetilamonijev klorid;DDAC;sekvencioniranje RNA;RNA-seq;biofilmi;izlivne črpalke;mehanizmi prilagoditve; |
Vrsta dela (COBISS): |
Članek v reviji |
Strani: |
str. 1-18 |
Letnik: |
ǂVol. ǂ8 |
Zvezek: |
ǂiss. ǂ3 |
Čas izdaje: |
2020 |
DOI: |
10.3390/microorganisms8030344 |
ID: |
14060819 |