Urška Ribič (Avtor), Jernej Jakše (Avtor), Nataša Toplak (Avtor), Simon Koren (Avtor), Minka Kovač (Avtor), Anja Klančnik (Avtor), Barbara Jeršek (Avtor)

Povzetek

Staphylococcus epidermidis cleanroom strains are often exposed to sub-inhibitory concentrations of disinfectants, including didecyldimethylammonium chloride (DDAC). Consequently, they can adapt or even become tolerant to them. RNA-sequencing was used to investigate adaptation and tolerance mechanisms of S. epidermidis cleanroom strains (SE11, SE18), with S. epidermidis SE11Ad adapted and S. epidermidis SE18To tolerant to DDAC. Adaptation to DDAC was identified with up-regulation of genes mainly involved in transport (thioredoxin reductase [pstS], the arsenic efflux pump [gene ID, SE0334], sugar phosphate antiporter [uhpT]), while down-regulation was seen for the Agr system (agrA, arC, agrD, psm, SE1543), for enhanced biofilm formation. Tolerance to DDAC revealed the up-regulation of genes associated with transporters (L-cysteine transport [tcyB]; uracil permease [SE0875]; multidrug transporter [lmrP]; arsenic efflux pump [arsB]); the down-regulation of genes involved in amino-acid biosynthesis (lysine [dapE]; histidine [hisA]; methionine [metC]), and an enzyme involved in peptidoglycan, and therefore cell wall modifications (alanine racemase [SE1079]). We show for the first time the differentially expressed genes in DDAC-adapted and DDAC-tolerant S. epidermidis strains, which highlight the complexity of the responses through the involvement of different mechanisms.

Ključne besede

mikrobiološka kontrola obratov;čisti prostori;patogeni mikroorganizmi;Staphylococcus epidermidis;razkuževanje;didecildimetilamonijev klorid;DDAC;sekvencioniranje RNA;RNA -seq;biofilmi;izlivne črpalke;mehanizmi prilagoditve;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL BF - Biotehniška fakulteta
UDK: 579.24+579.26:613.6
COBISS: 5173368 Povezava se bo odprla v novem oknu
ISSN: 2076-2607
Št. ogledov: 143
Št. prenosov: 64
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: mikrobiološka kontrola obratov;čisti prostori;patogeni mikroorganizmi;Staphylococcus epidermidis;razkuževanje;didecildimetilamonijev klorid;DDAC;sekvencioniranje RNA;RNA-seq;biofilmi;izlivne črpalke;mehanizmi prilagoditve;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-18
Letnik: ǂVol. ǂ8
Zvezek: ǂiss. ǂ3
Čas izdaje: 2020
DOI: 10.3390/microorganisms8030344
ID: 14060819