Anja Krajnc (Avtor), Aljaž Gaber (Avtor), Brigita Lenarčič (Avtor), Miha Pavšič (Avtor)

Povzetek

Testicans are modular proteoglycans of the extracellular matrix of various tissues where they contribute to matrix integrity and exert cellular effects like neurite outgrowth and cell migration. Using testican-2 as a representative member of the family, we tackle the complete lack of general structural information and structure–function relationship. First, we show using isothermal titration calorimetry and modeling that extracellular calcium-binding domain (EC) has only one active calcium-binding site, while the other potential site is inactive, and that testican-2 is within extracellular matrix always in the calcium-loaded form. Next, we demonstrate using various prediction methods that N- and C-terminal regions plus interdomain connections are flexible. We support this by small-angle X-ray-scattering analysis of C-terminally truncated testican-2, which indicates that the triplet follistatin-EC-thyroglobulin domain forms a moderately compact core while the unique N-terminal is disordered. Finally, using cell exclusion zone assay, we show that it is this domain triplet that is responsible for promoting cell migration and not the N- and C-terminal regions.

Ključne besede

testikan;SPOCK;vezava kalcijevih ionov;model strukture;celična migracija;testican;calcium-binding;structural model;cell migration;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL FKKT - Fakulteta za kemijo in kemijsko tehnologijo
UDK: 577.112.85
COBISS: 42261507 Povezava se bo odprla v novem oknu
ISSN: 1661-6596
Št. ogledov: 248
Št. prenosov: 87
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: testikan;SPOCK;vezava kalcijevih ionov;model strukture;celična migracija;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-17
Letnik: ǂVol. ǂ21
Zvezek: ǂiss. ǂ24
Čas izdaje: Dec. 2020
DOI: 10.3390/ijms21249413
ID: 14444840