Chemokines during anaphylaxis

ǂthe ǂimportance of CCL2 and CCL2-dependent chemotactic activity for basophils

Romana Vantur (Avtor), Maruša Rihar (Avtor), Ana Koren (Avtor), Matija Rijavec (Avtor), Peter Kopač (Avtor), Urška Bidovec (Avtor), Renato Eržen (Avtor), Peter Korošec (Avtor)

Povzetek

Background: The role of chemokines in anaphylaxis is unclear. Methods: We prospectively recruited 49 patients presenting to the emergency department with an acute episode of anaphylaxis and 28 healthy subjects. We measured serum levels of the chemokines CCL2, CCL5, CCL7, CCL8, CCL11, CCL13, CCL17, CCL21, CCL22, CCL24, and CCL26, tryptase, the absolute number of circulating basophils, monocytes, lymphocytes, and PMNs, and whole blood FCER1A, CPA3 and HDC gene expression at two time points: during the anaphylactic episode and in convalescent samples collected approximately 3 months later. We then investigated the in vitro chemotactic activity of chemokines induced during anaphylaxis for the in vitro migration of the corresponding cells. Results: Only CCL2 chemokine levels were signifcantly increased in anaphylaxis samples (median 514 pg/ml) compared to convalescent samples (284 pg/ml, P<0.0001) and healthy subjects (279 pg/ml, P<0.0001); there was no signifcant diference in any of the other chemokines. There was a signifcant positive correlation between the rates of increase of serum CCL2 (median [range]: 106.0% [-44.7% to 557.4%]) and tryptase (133.8% [-6.6% to 893.4%]; r=0.68, P<0.0001) and between the acute concentration of serum CCL2 and the acute concentration of serum tryptase (r=0.77, P<0.0001). The number of circulating basophils, but not other blood cells, signifcantly decreased during anaphylaxis (median 5.0 vs. 19.1 cells/[micro]l in convalescent samples; P<0.0001); a decrease in whole-blood gene expression of basophil markers (P

Ključne besede

CCL2;celična migracija;cell migration;

Podatki

Jezik:	Angleški jezik
Leto izida:	2020
Tipologija:	1.01 - Izvirni znanstveni članek
Založnik:	Springer Nature
UDK:	616-097
COBISS:	43048963
ISSN:	2045-7022
Št. ogledov:	817
Št. prenosov:	333
Ocena:	0 (0 glasov)
Metapodatki:

Ostali podatki

Sekundarne ključne besede:	Anaphylaxis;Chemokines;Tryptases;Basophils;Chemotaxis;Anafilaksija;Kemokini;Triptaze;Bazofilci;Kemotaksa;
Komentar vira:	Nasl. z nasl. zaslona; Soavtorji: Maruša Rihar, Ana Koren, Matija Rijavec, Peter Kopač, Urška Bidovec-Stojković, Renato Eržen, Peter Korošec; Opis vira z dne 16. 12. 2020; Št. članka: 63;
Strani:	str. 1-11
Zvezek:	ǂVol. ǂ10
Čas izdaje:	2020
DOI:	10.1186/s13601-020-00367-2
ID:	12400276