Pulsed electric field (PEF) enhances iron uptake by the yeast Saccharomyces cerevisiae

Karolina Nowosad (Avtor), Monika Sujka (Avtor), Urszula Pankiewicz (Avtor), Damijan Miklavčič (Avtor), Marta Arczewska (Avtor)

Povzetek

The aim of the study was to investigate the influence of a pulsed electric field (PEF) on the level of iron ion accumulation in Saccharomyces cerevisiae cells and to select PEF conditions optimal for the highest uptake of this element. Iron ions were accumulated most efficiently when their source was iron (III) nitrate. When the following conditions of PEF treatment were used: voltage 1500 V, pulse width 10 µs, treatment time 20 min, and a number of pulses 1200, accumulation of iron ions in the cells from a 20 h-culture reached a maximum value of 48.01 mg/g dry mass. Application of the optimal PEF conditions thus increased iron accumulation in cells by 157% as compared to the sample enriched with iron without PEF. The second derivative of the FTIR spectra of iron-loaded and -unloaded yeast cells allowed us to determine the functional groups which may be involved in metal ion binding. The exposure of cells to PEF treatment only slightly influenced the biomass and cell viability. However, iron-enriched yeast (both with or without PEF) showed lower fermentative activity than a control sample. Thus obtained yeast biomass containing a high amount of incorporated iron may serve as an alternative to pharmacological supplementation in the state of iron deficiency.

Ključne besede

kopičenje železa;kvas;pomanjkanje železa;PEF;iron accumulation;yeast;iron deficiency;

Podatki

Jezik:	Angleški jezik
Leto izida:	2021
Tipologija:	1.01 - Izvirni znanstveni članek
Organizacija:	UL FE - Fakulteta za elektrotehniko
UDK:	577:621.3
COBISS:	66394115
ISSN:	2218-273X
Št. ogledov:	82
Št. prenosov:	45
Ocena:	0 (0 glasov)
Metapodatki:

Ostali podatki

Sekundarni jezik:	Slovenski jezik
Sekundarne ključne besede:	kopičenje železa;kvas;pomanjkanje železa;PEF;
Vrsta dela (COBISS):	Članek v reviji
Strani:	str. 1-19
Letnik:	ǂiss. ǂ6
Zvezek:	850
Čas izdaje:	Jun. 2021
DOI:	10.3390/biom11060850
ID:	14825820