Manca Žerovnik (Avtor), Ciril Bohak (Avtor), Eva Boneš (Avtor), Samo Hudoklin (Avtor), Rok Romih (Avtor), Matija Marolt (Avtor)

Povzetek

Background and objectives: In recent years, electron microscopy is enabling the acquisition of volumetric data with resolving power to directly observe the ultrastructure of intracellular compartments. New insights and knowledge about cell processes that are offered by such data require a comprehensive analysis which is limited by the time-consuming manual segmentation and reconstruction methods. Method: We present methods for automatic segmentation, reconstruction, and analysis of intracellular compartments from volumetric data obtained by the dual-beam electron microscopy. We specifically address segmentation of fusiform vesicles and the Golgi apparatus, reconstruction of mitochondria and fusiform vesicles, and morphological analysis of the reconstructed mitochondria. Results and conclusion: Evaluation on the public UroCell dataset demonstrated high accuracy of the proposed methods for segmentation of fusiform vesicles and the Golgi apparatus, as well as for reconstruction of mitochondria and analysis of their shapes, while reconstruction of fusiform vesicles proved to be more challenging. We published an extension of the UroCell dataset with all of the data used in this work, to further contribute to research on automatic analysis of the ultrastructure of intracellular compartments.

Ključne besede

mikroskopija;mitohondriji;Golgijev aparat;fuziformni vezikli;urotelij;globoko učenje;reconstruction;instance segmentation;electron microscopy;mitochondria;Golgi apparatus;fusiform vesicles;urothelium;deep learning;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL FRI - Fakulteta za računalništvo in informatiko
UDK: 004.8:537.533.35
COBISS: 112962819 Povezava se bo odprla v novem oknu
ISSN: 0169-2607
Št. ogledov: 90
Št. prenosov: 46
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: mikroskopija;mitohondriji;Golgijev aparat;fuziformni vezikli;urotelij;globoko učenje;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-14
Zvezek: ǂVol. ǂ223
Čas izdaje: Aug. 2022
DOI: 10.1016/j.cmpb.2022.106959
ID: 15776737