Mojca Pavlin (Avtor), Jasna Lojk (Avtor), Klemen Strojan (Avtor), Iva Hafner Bratkovič (Avtor), Roman Jerala (Avtor), Adrijana Leonardi (Avtor), Igor Križaj (Avtor), Nataša Drnovšek (Avtor), Saša Novak (Avtor), Peter Veranič (Avtor), Vladimir Boštjan Bregar (Avtor)

Povzetek

Alongside physiochemical properties (PCP), it has been suggested that the protein corona of nanoparticles (NPs) plays a crucial role in the response of immune cells to NPs. However, due to the great variety of NPs, target cells, and exposure protocols, there is still no clear relationship between PCP, protein corona composition, and the immunotoxicity of NPs. In this study, we correlated PCP and the protein corona composition of NPs to the THP-1 macrophage response, focusing on selected toxicological endpoints: cell viability, reactive oxygen species (ROS), and cytokine secretion. We analyzed seven commonly used engineered NPs (SiO$_2$, silver, and TiO$_2$) and magnetic NPs. We show that with the exception of silver NPs, all of the tested TiO$_2$ types and SiO$_2$ exhibited moderate toxicities and a transient inflammatory response that was observed as an increase in ROS, IL-8, and/or IL-1β cytokine secretion. We observed a strong correlation between the size of the NPs in media and IL-1β secretion. The induction of IL-1β secretion was completely blunted in NLR family pyrin domain containing 3 (NLRP3) knockout THP-1 cells, indicating activation of the inflammasome. The correlations analysis also implicated the association of specific NP corona proteins with the induction of cytokine secretion. This study provides new insights toward a better understanding of the relationships between PCP, protein corona, and the inflammatory response of macrophages for different engineered NPs, to which we are exposed on a daily basis.

Ključne besede

fizikalno-kemijske lastnosti;nanodelci;nanotoksikologija;imunski odziv;korelacija;proteinska korona;citokini;inflamasom;makrofagi;TiO2;physico-chemical properties;nanoparticles;nanotoxicology;immune response;correlation;protein corona;cytokines;inflammasome;macrophages;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL FE - Fakulteta za elektrotehniko
UDK: 577
COBISS: 109968387 Povezava se bo odprla v novem oknu
ISSN: 1422-0067
Št. ogledov: 19
Št. prenosov: 5
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: fizikalno-kemijske lastnosti;nanodelci;nanotoksikologija;imunski odziv;korelacija;proteinska korona;citokini;inflamasom;makrofagi;TiO$_2$;
Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-23
Letnik: ǂiss. ǂ11
Zvezek: 6197
Čas izdaje: Jun.-1 2022
DOI: 10.3390/ijms23116197
ID: 18583951