Nastja Sluga (Avtor), Sandra Postić (Avtor), Srdjan Sarikas (Avtor), Ya-Chi Huang (Avtor), Andraž Stožer (Avtor), Marjan Rupnik (Avtor)

Povzetek

Cholinergic innervation in the pancreas controls both the release of digestive enzymes to support the intestinal digestion and absorption, as well as insulin release to promote nutrient use in the cells of the body. The effects of muscarinic receptor stimulation are described in detail for endocrine beta cells and exocrine acinar cells separately. Here we describe morphological and functional criteria to separate these two cell types in situ in tissue slices and simultaneously measure their response to ACh stimulation on cytosolic Ca2+ oscillations [Ca2+]c in stimulatory glucose conditions. Our results show that both cell types respond to glucose directly in the concentration range compatible with the glucose transporters they express. The physiological ACh concentration increases the frequency of glucose stimulated [Ca2+]c oscillations in both cell types and synchronizes [Ca2+]c oscillations in acinar cells. The supraphysiological ACh concentration further increases the oscillation frequency on the level of individual beta cells, inhibits the synchronization between these cells, and abolishes oscillatory activity in acinar cells. We discuss possible mechanisms leading to the observed phenomena.

Ključne besede

pancreas tissue slices;acetylcholine;beta cell;acinar cell;Ca2+ oscillations;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UM MF - Medicinska fakulteta
Založnik: MDPI
UDK: 612.349.7
COBISS: 68553731 Povezava se bo odprla v novem oknu
ISSN: 2073-4409
Št. ogledov: 0
Št. prenosov: 8
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Slovenski jezik
Sekundarne ključne besede: tkivne rezine trebušne slinavke;acetilholin;beta celice;acinarne celice;kalcijeve oscilacije;
Vrsta dela (COBISS): Znanstveno delo
Strani: str. 1-14
Zvezek: ǂVol.ǂ10
Čas izdaje: 2021
DOI: 10.3390/cells10071580
ID: 25290851