Martin Fischlechner (Avtor), Markus Zaulig (Avtor), Stefan Meyer (Avtor), Irina Estrela-Lopis (Avtor), Luis Cuéllar (Avtor), Joseba Irigoyen (Avtor), Paula Pescador (Avtor), Milan Brumen (Avtor), Paul Messner (Avtor), Sergio Moya (Avtor), Edwin Donath (Avtor)

Povzetek

The mechanism of formation of supported lipid layers from phosphatidylcholine and phosphatidylserine vesicles in solution on polyelectrolyte multilayers was studied by a variety of experimental techniques. The interaction of zwitterionic and acidic lipid vesicles, as well as their mixtures, with polyelectrolyte supports was followed in real time by micro-gravimetry. The fabricated lipid-polyelectrolyte composite structures on top of multilayer coated colloidal particles were characterized by flow cytometry and imaging techniques. Lipid diffusion over the macroscopic scale was quantified by fluorescence recovery after photobleaching, and the diffusion was related to layer connectivity. The phospholipid-polyelectrolyte binding mechanism was investigated by infrared spectroscopy. A strong interaction of polyelectrolyte primary amino groups with phosphate and carboxyl groups of the phospholipids, leading to dehydration, was observed. Long-range electrostatic attraction was proven to be essential for vesicle spreading and rupture. Fusion of lipid patches into a homogeneous bilayer required lateral mobility of the lipids on the polyelectrolyte support. The binding of amino groups to the phosphate group of the zwitterionic lipids was too weak to induce vesicle spreading, but sufficient for strong adsorption. Only the mixture of phosphatidylcholine and phosphatidylserine resulted in the spontaneous formation of bilayers on polyelectrolyte multilayers. The adsorption of phospholipids onto multilayers displaying quarternary ammonium polymers produced a novel 3D lipid polyelectrolyte structure on colloidal particles.

Ključne besede

biofizika;lipidi;lipidne plasti;elektroliti;biophysics;lipids;lipid layers;electrolytes;multilayer supports;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UM FNM - Fakulteta za naravoslovje in matematiko
UDK: 577.115:53.082.75
COBISS: 16347912 Povezava se bo odprla v novem oknu
ISSN: 1744-683X
Št. ogledov: 1109
Št. prenosov: 58
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Angleški jezik
Sekundarne ključne besede: biofizika;lipidi;lipidne plasti;elektroliti;
URN: URN:SI:UM:
Strani: str. 2245-2258
Letnik: 4
Zvezek: ǂiss. ǂ11
Čas izdaje: 2008
DOI: 10.1039/b805754k
ID: 8724044