developing an assay for screening anti-inflammatory milk proteins and peptides
Gorazd Tompa (Avtor), Aire Laine (Avtor), Anne Pihlanto (Avtor), Hannu Korhonen (Avtor), Irena Rogelj (Avtor), Pertti Marnila (Avtor)

Povzetek

Human leukemic THP-1 promonocytes are widely used as a model for peripheral blood monocytes. However, superoxide production during respiratory burst (RB) of non-differentiated THP-1 (nd-THP-1) cells is very low. Here we present a rapid and low-cost method for measuring the chemiluminescence (CL) of opsonized zymosan (OZ) induced RB which allows detection of Escherichia coli lipopolysaccharide (LPS) induced priming of nd-THP-1 cells on the basis of CL reaction kinetics. Maximum CL intensity obtained was 2.20+- 0.25 and 1.30+- 0.11 relative light units, while CL peak time was achieved at 18.1+- 2.6 and 28.7+- 1.3 min in primed and non-primed cells, respectively. The priming of nd-THP-1 cells with LPS evoked tzpical TNF-[alfa] and IL-6 production. We tested the effects of bovine lactoferrin and protein fractions from Lactobacillus helveticus BGRA43 fermented milk for potential anti-inflammatory effects on LPS primed nd-THP-1 cells. Four fractions were found to inhibit the OZ-induced CL in a dose-dependent manner (IC50 3-30 g/mL), while lactoferrin inhibited CL to a lesser extent (IC50 270? g/mL). These results suggest that measuring CL response of nd-THP-1 cells can serve as a method for screening anti-inflammatory compounds which could be used in reducing the risk of phagocyte-mediated inflammatory diseases.

Ključne besede

mleko;beljakovine;peptidi;analizna kemija;kemiluminiscenca;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL BF - Biotehniška fakulteta
UDK: 637.1:543
COBISS: 2757768 Povezava se bo odprla v novem oknu
ISSN: 1522-7235
Št. ogledov: 1310
Št. prenosov: 215
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Neznan jezik
Vrsta dela (COBISS): Delo ni kategorizirano
Strani: str. 251-258
Letnik: ǂVol. ǂ26
Zvezek: ǂno. ǂ4
Čas izdaje: 2011
DOI: 10.1002/bio.1220
ID: 1033738
Priporočena dela:
, developing an assay for screening anti-inflammatory milk proteins and peptides
, diplomsko delo
, ni podatka o podnaslovu
, zbrano gradivo
, diplomsko delo visokošolskega študija