magistrsko delo
Saša Rešeta (Avtor), Sašo Gyergyek (Mentor), David Pahovnik (Komentor)

Povzetek

Stabilne suspenzije magnetnih nanodelcev prevlečenih z bioaktivnimi molekulami predstavljajo velik potencial v terapevtskih aplikacijah. Goli magnetni nanodelci v vodnih suspenzijah imajo težnjo po aglomeraciji, z adsorpcijo aminokislin nanje pa lahko vplivamo na njihov površinski naboj in tako pripravimo stabilne suspenzije. V magistrskem delu smo želeli pripraviti stabilne suspenzije magnetnih nanodelcev maghemita prevlečenih s polipeptidom na enostaven in cenovno ugoden način, primeren za masovno industrijsko proizvodnjo. Preučevali smo tudi obstojnost peptidne prevleke v biološko pomembnem mediju, fosfatnem pufru, ki ima veliko afiniteto do površine nanodelca. Magnetne nanodelce smo sintetizirali s soobarjanjem Fe2+/Fe3+ ionov iz vodne raztopine. Na tako pripravljene nanodelce smo adsorbirali aminokislini lizin in asparaginsko kislino v različnih razmerjih. Za zamreženje adsorbiranih aminokislin smo uporabili zamreževalni reagent 1-etil-3-(-3-dimetilaminopropil) karbodiimid hidroklorid (EDC), ki je znan po nizki toksičnosti in sposobnosti modifikacije stranskih skupin proteinov. Z adsorpcijo aminokislin na površino magnetnih nanodelcev in naknadnim dodatkom zamreževalnega reagenta nismo uspeli pripraviti koloidno stabilnih suspenzij. S FT-IR meritvami smo potrdili nastanek peptida na površini magnetnih nanodelcev. Na podlagi HPLC meritev aminokislin v supernatantih suspenzij nanodelcev sklepamo, da se na nanodelce v prisotnosti EDC adsorbira precejšen del aminokislin, pri čemer se asparaginska kislina adsorbira v večji meri kot lizin. Meritve zeta potenciala so pokazale obstojnost peptidne prevleke na nanodelcih v prisotnosti fosfatnega pufra s koncentracijo, ki se uporablja v bioloških aplikacijah.

Ključne besede

magnetni nanodelci;maghemit;asparaginska kislina;lizin;magistrske naloge;

Podatki

Jezik: Slovenski jezik
Leto izida:
Tipologija: 2.09 - Magistrsko delo
Organizacija: UM FKKT - Fakulteta za kemijo in kemijsko tehnologijo
Založnik: [S. Rešeta]
UDK: 620.3:665.7.035.2(043.2)
COBISS: 21030422 Povezava se bo odprla v novem oknu
Št. ogledov: 983
Št. prenosov: 106
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Angleški jezik
Sekundarni naslov: Colloidal properties of magnetic nanoparticles coated by polypeptides
Sekundarni povzetek: Stable suspensions of magnetic nanoparticles coated with bioactive molecules show great potential for therapeutic applications. Magnetic nanoparticles in aqueous suspensions have the tendency to agglomerate, and with their adsorption of amino acids we can influence their surface charge, thus preparing stable suspensions. In this Master’s thesis, we aimed to prepare stable suspensions of maghemite magnetic nanoparticles coated with a polypeptide in a simple and cost-effective manner suitable for mass industrial production. We also researched the stability of a peptide coating in a biologically important medium, a phosphate buffer, which has a great affinity for the surface of the nanoparticle. The magnetic nanoparticles were synthesized through the co-precipitation of the Fe2+/Fe3+ ions in the aqueous solution. Once the nanoparticles were prepared, we adsorbed the amino acids lysine and aspartic acid in varying proportions. In order to cross-link the adsorbed amino acids we used the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) cross-linking reagent, which is known for its low toxicity and its ability to modify side groups of proteins. With the adsorption of the amino acids onto the surface of the magnetic nanoparticles and the subsequent addition of the cross-linking reagent, we were unable to prepare colloidally stable suspensions. FT-IR measurements confirmed the formation of a peptide on the surface of magnetic nanoparticles. Based on our HPLC measurements of amino acids in supernatant nanoparticle suspensions, we can conclude that in the presence of EDC nanoparticles adsorb a substantial portion of the amino acids, of which aspartic acid is adsorbed to a greater extent than lysine. Our measurements of the zeta potential demonstrated the persistence of the peptide coating on the nanoparticles in the presence of a phosphate buffer at a concentration used in biological applications.
Sekundarne ključne besede: magnetic nanoparticles;maghemite;aspartic acid;lysine;EDC;PBS;
URN: URN:SI:UM:
Vrsta dela (COBISS): Magistrsko delo/naloga
Komentar na gradivo: Univ. v Mariboru, Fak. za kemijo in kemijsko tehnologijo
Strani: XIII, 57 str.
ID: 10863811