Luca Rivoira (Avtor), Mojca Žorž (Avtor), Mitja Martelanc (Avtor), Sara Budal (Avtor), Davide Carena (Avtor), Mladen Franko (Avtor), Maria Concetta Bruzzoniti (Avtor)

Povzetek

In this work, new analytical approaches for determination of biogenic amines in wines were developed. For the first time, we studied the derivatization of BAs in wines with naphthalene- 2,3-dicarboxaldehyde (NDA) and with dabsyl chloride (DBS) and analysis of derivatized BAs by HPLC coupled to fluorescence (HPLC-NDA-FL) and thermal lens spectrometry (HPLC-DBS-TLS) detectors. The sensitivity of the two methods (LODs HPLC-NDA-FL in the range 27-73 μg/L; LODs HPLC-DBS-TLS in the range 3.4-11 μg/L) was higher than that of the official method for biogenic amines in wines, OIV-MA-AS315-18 (60-77 μg/L). For its best performances, the HPLC-DBS-TLS technique was applied to the analysis of putrescine, cadaverine, histamine and tyramine in two white wine samples. Additionally, exploiting the Berthelot reaction, the TLS fast screening of biogenic amines in wines, following the release of ammonia by transglutaminase, was also proposed. This approach allowed us to determine total biogenic amount content in concentrations below 0.1 mg/L, expressed as equivalents of histamine.

Ključne besede

biogenic amines;NDA;liquid chromatography;fluorescence;wine;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UNG - Univerza v Novi Gorici
UDK: 54
COBISS: 4945915 Povezava se bo odprla v novem oknu
ISSN: 1224-7154
Št. ogledov: 3787
Št. prenosov: 264
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

URN: URN:SI:UNG
Vrsta dela (COBISS): Delo ni kategorizirano
Strani: str. 103-122
Letnik: ǂVol. ǂ62
Zvezek: ǂiss. ǂ3
Čas izdaje: 2017
DOI: 10.24193/subbchem.2017.3.08
ID: 10874550