primer severne linejevke (Linnaea borealis)
Tea Kelc (Avtor), Nataša Pipenbaher (Mentor), Mitja Kaligarič (Komentor), Metka Šiško (Komentor)

Povzetek

Rastline, ki so omejene na majhne in izolirane populacije, so bolj občutljive na podnebne, življenjske in biogenetske dejavnike. V raziskavo smo vključili dve majhni izolirani populaciji severne linejevke (Linnaea borealis L.) iz vzhodnih Alp in eno populacijo iz osrednjega borealnega območja na Švedskem. Namen magistrskega dela je z uporabo mikrosatelitskih markerjev dokazati, da je rastlinska vrsta (L. borealis) na omenjenih območjih prisotna v več klonih. Ugotavljali smo tudi, ali je genetska variabilnost obeh alpskih izoliranih populacij iz Slovenije in Avstrije zmanjšana glede na borealno populacijo iz Skandinavije. Na devetih mikrosatelitnih lokusih A5, A102, D100a, D7, D110, D118, A112, B119 in C105 smo pri 45 vzorcih (15 iz vsakega območja) skupaj namnožili 70 alelov, v povprečju 7,78 alela na lokus. Genetske razdalje analiziranih genotipov smo izračunali z Diceovim koeficientom in izdelali dendrogram ter multivariatno analizo. Ugotovili smo zelo nizke genetske razlike v obeh izoliranih populacijah in zelo visoke genetske razlike med obema populacijama, čeprav je med njima le 73 km zračne razdalje. Sklepamo, da sta zaradi visoke genetske variabilnosti med njima obe izolirani alpski populaciji glacialna relikta, najverjetneje zaradi zaporednih ozkih grl in dolgoročne izolacije v posebnih okoljskih pogojih. Izolirani populaciji L. borealis nista popolnoma izgubili svoje sposobnosti spolnega razmnoževanja, zato je "in-situ" preživetje še zmeraj mogoče. Vendar pa so glacialni relikti običajno povezani z ranljivimi habitati, ki si zaslužijo nujno pozornost zaradi preživetja z ohranjanjem in s posebnimi protiukrepi, zlasti z vidika globalnega segrevanja.

Ključne besede

magistrska dela;glacialni relikt;mikrosateliti;molekulski markerji;razpršena porazdelitev območja;vzhodne Alpe;

Podatki

Jezik: Slovenski jezik
Leto izida:
Tipologija: 2.09 - Magistrsko delo
Organizacija: UM FNM - Fakulteta za naravoslovje in matematiko
Založnik: [T. Kelc]
UDK: 575.16:581.1(043.2)
COBISS: 23795720 Povezava se bo odprla v novem oknu
Št. ogledov: 990
Št. prenosov: 101
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Angleški jezik
Sekundarni naslov: Influences of genetic impoverishment on survival ability of small populations of rare plants
Sekundarni povzetek: Plants occurring under small and isolated populations are more vulnerable to climatic, habitat and biogenetic factors. We studied two small isolated populations of twinflower (Linnaea borealis L.) from the Eastern Alps and one population from the core Boreal distributional range in Sweden. The purpose of the thesis was, using microsatellite markers, to prove that the plant species (L. borealis) is in the Eastern Alps region present in several clones. We also examined whether the genetic variability of both alpine isolated populations from Slovenia and Austria was reduced due to the boreal population from Scandinavia. Nine microsatellite loci (A5, A102, D100a, D7, D110, D118, A112, B119, C105) were taken into account, 45 samples were examinated (15 from each area) and 70 alleles were exhibited with an average of 7.78 alleles per loci. The genetic distances of the analyzed genotypes were calculated by Dice coefficient to form a dendrogram and multivariate analysis. We found very low genetic variability in both isolated populations and very high genetic variability between the two populations, despite there is only 73 km of air distance between them. We conclude that because of the high genetic variability between them, both isolated alpine populations are glacial relict populations, probably due to consecutive bottlenecks and long-term isolation in particular environmental conditions. The isolated populations of the L. borealis in the Eastern Alps have not completely lost their sexual reproduction capacity, so "in-situ" survival is still possible. However, glacial relics are usually associated with vulnerable habitats which deserve urgent attention to survive involving conservation and special countermeasures especially in terms of global warming.
Sekundarne ključne besede: master theses;glacial relict;microsatellites;molecular markers;disjunct distributional range;Eastern Alps;
URN: URN:SI:UM:
Vrsta dela (COBISS): Magistrsko delo/naloga
Komentar na gradivo: Univ. v Mariboru, Fak. za naravoslovje in matematiko, Oddelek za biologijo
Strani: VIII f., 40 str.
ID: 10920408