Jezik: | Slovenski jezik |
---|---|
Leto izida: | 2018 |
Tipologija: | 2.09 - Magistrsko delo |
Organizacija: | UL BF - Biotehniška fakulteta |
Založnik: | [E. Uranič] |
UDK: | 602.3+602.6:579.873.7:577.1 |
COBISS: | 4930680 |
Št. ogledov: | 1338 |
Št. prenosov: | 537 |
Ocena: | 0 (0 glasov) |
Metapodatki: |
Sekundarni jezik: | Angleški jezik |
---|---|
Sekundarni naslov: | Heterologus expression of genes for terpenoide biosynthetic pathways in selected Streptomyces sp. strains |
Sekundarni povzetek: | Bacteria of genus Streptomyces are well known secondary metabolites producers. Streptomyces species are also attractive host for heterologous production of various metabolites in industrial level. The aim of our thesis was to express genes for biosynthesis of isopentenyl diphosphate (IPP) and genes for conversion of IPP in βcatotene in Streptomyces sp. Plasmids with mevalonate (MEV) and with β-carotene pathway were constructed. Genes for MEV pathway were extracted from two Streptomyces species; S. cinnamonensis (MEVC) and K. griseola (MEVK). Genes for β- carotene biosynthesis were used from bacteria Erwinia uredova. For plasmid construction we used integrative vectors with phiBT and phiC31 virus integrase and E. coli-Streptomyces replicative vector pVF. We engineered plasmids with MEV pathway and transformed them into S. rimosus successfully. We also try to transform plasmids with β-carotene pathway but were unsuccesful. |
Sekundarne ključne besede: | Streptomyces rimosus;heterologous expression;biosynthesis;[beta]-caroten;mevalonat pathway;deoxysilulose-5-phosphate pathway;isopentenyl diphosphate;plasmid; |
Vrsta dela (COBISS): | Magistrsko delo/naloga |
Študijski program: | 0 |
Komentar na gradivo: | Univ. v Ljubljani, Biotehniška fak., Študij mikrobiologije |
Strani: | XIV, 76 str., [6] str. pril. |
ID: | 10944623 |