Elisa Mazzega (Avtor), Ario De Marco (Avtor), Marina Cabrini (Avtor), Alfred Beran (Avtor)

Povzetek

At the present, the identification of planktonic species in coastal water mainly relies on light microscopy observations. This kind of analyses is performed by highly trained personnel, requires lab equipment and long processing time. High-throughput and easy-to-perform methods are instead highly needed for routine costal and ballast water monitoring. Immuno-reagents are widely employed in the medical field for routine diagnostics, where they provide the necessary sensitivity and specificity, as for example for cancer subtype characterization. Reagents of similar grade are so far not widely available for both diagnostics and basic research of microalgae. We describe the first successful isolation of a single-domain antibody (nanobody or VHH) from a pre-immune library, its engineering into application-ready reagents, and its inexpensive production as recombinant fusion protein. Alexandrium minutum was chosen as a model organism to test the feasibility of the procedure. The procedure foresees the panning of a pre-immune phage library of VHHs that was used for in vitro selection against directly the target cells. Monoclonal nanobodies specific for A. minutum cells were identified and optimized for recombinant production as fusion with fluorescent proteins in bacterial hosts. Such fluorescently-tagged VHHs were validated by immunofluorescence and cytofluorimetry for their selectivity by testing unicellular algal species that can be found in the same environment of A. minutum. Two nanobodies were found to be highly specific for the target cells, were able to bind also cysts of A. minutum and they gave no cross-reaction, even for a not-toxic strain of the closely related A. tamutum. Different tags can be then fused to the selected nanobodies and used instead of the fluorescent proteins to obtain a reagent immediately applicable to further techniques, such as cell Enzyme Linked Immuno Sorbent Assay (ELISA) or biosensor surface functionalization. The newly produced reagents can be applied for direct whole-cell detection in seawater, bypassing the need of cell processing required for DNA or RNA diagnostics, and can be used for both alive and fixed cells, guaranteeing the possibility to check old samples and to perform confirmatory morphological studies.

Ključne besede

nanobodies;toxic algae;recombinant reagents;naive library;phage display;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.12 - Objavljeni povzetek znanstvenega prispevka na konferenci
Organizacija: UNG - Univerza v Novi Gorici
UDK: 577
COBISS: 5303803 Povezava se bo odprla v novem oknu
Št. ogledov: 2979
Št. prenosov: 0
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

URN: URN:SI:UNG
Vrsta dela (COBISS): Delo ni kategorizirano
Strani: Str. 553
ID: 10994965
Priporočena dela:
, flexible structures for biotechnological applications
, ǂa ǂuniversal synthetic library of humanized nanobodies providing highly functional antibodies and intrabodies
, lecture at Università degli Studi di Milano, Dipartimento di Scienze Farmacologiche e Biomolecolari, 11. 10. 2018