magistrsko delo
Ines Jerovšek (Avtor), Uroš Petrovič (Recenzent), Neža Čadež (Mentor), Lorena Butinar (Komentor)

Povzetek

Najpomembnejša kvasovka v biotehnologiji je Saccharomyces cerevisiae, ki si primarno ekološko nišo deli z ozko sorodno vrsto Saccharomyces paradoxus. V magistrskem delu smo želeli ohraniti genetski potencial 386 sevov kvasovk Vipavskega vinorodnega okoliša in določiti njihovo genetsko raznolikost. Šest sevov kvasovk smo shranili pri –80 °C in ob tem določili njihovo stopnjo preživetja po enem in štirih mesecih. Stopnja preživetja kvasovk je s časom upadala. Najvišjo stopnjo preživetja so imele kvasovke Dekkera bruxellensis, S. cerevisiae, Metschnikowia pulcherrima in Rhodotorula glutinis. Na podlagi rezultatov PCR-RFLP regije ITS smo razlikovali S. cerevisiae in S. paradoxus od drugih vrst kvasovk. Izmed 204 Saccharomyces sevov je bilo 5 % izolatov S. cerevisiae in 9 % S. paradoxus iz naravnega okolja, ostali so bili iz vinograda. Ostale vrste kvasovk rodov Candida, Debaryomyces, Hanseniaspora, Kluyveromyces, Metschnikowia, Meyerozyma, Lachancea, Pichia, Schwanniomyces, Torulaspora in Zygosaccharomyce smo identificirali s sekvenciranjem regije ITS, ter jim določili genetsko raznolikost na podlagi vzorca pomnožkov z (GTG)5x. S povezovanjem genetske raznolikosti v mikrosatelitskih hipervariabilnih regijah vrst Saccharomyces in njihovim fenotipom smo pokazali obstoj dveh skupin vinogradniških sevov S. cerevisiae, prve odporne proti visokim koncentracijam etanola in glukoze, druga pa protu kalijevemu metabisulfitu.

Ključne besede

kvasovke;Saccharomyces cerevisiae;Primorski vinorodni okoliš;krioprezervacija;stopnja preživetja;PCR-RFLP;ITS;(GTG)5X;mikrosateliti;

Podatki

Jezik: Slovenski jezik
Leto izida:
Tipologija: 2.09 - Magistrsko delo
Organizacija: UL BF - Biotehniška fakulteta
Založnik: [I. Jerovšek]
UDK: 579.25:582.282.23:577.2.08
COBISS: 5050744 Povezava se bo odprla v novem oknu
Št. ogledov: 974
Št. prenosov: 203
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Angleški jezik
Sekundarni naslov: Preservation of yeasts genetic potential from Primorska wine region and their genetic typing
Sekundarni povzetek: The most important yeast in biotechnology is Saccharomyces cerevisiae, which shares its primary ecological niche with a closely related species Saccharomyces paradoxus. The purpose of our work was to preserve genetic potential of 386 yeast strains isolated in Vipava valley and to determine their genetic diversity. Six yeast strains were preserved at –80 °C and their survival rate was determined after one and four months. Yeasts survival rate declined over time. The highest survival rates were found in species Dekkera bruxellensis, S. cerevisiae, Metschnikowia pulcherrima and Rhodotorula glutinis. Based on PCR-RFLP results of the ITS region we could differentiate S. cerevisiae and S. paradoxus from other yeast species. Five and nine percent of S. paradoxus and S. cerevisiae out of 204 isolates, respectively, were of non-vineyard origin. Other yeast species of genera Candida, Debaryomyces, Hanseniaspora, Kluyveromyces, Metschnikowia, Meyerozyma, Lachancea, Pichia, Schwanniomyces, Torulaspora and Zygosaccharomyce were identified by sequencing of the ITS region and their genetic diversity was determined by (GTG)5x fingerprinting. By combining genetic diversity in microsatellite hypervariable regions of the Saccharomyces species and their phenotypes, we determined two distinct groups of S. cerevisiae vineyard strains, one resistant to high concentration of ethanol and glucose, and the second to potassium metabisulphite.
Sekundarne ključne besede: yeasts;Saccharomyces cerevisiae;Primorska wine region;kriopreservation;survival rate;PCR-RFLP;ITS;(GTG)5X;microsatelites;
Vrsta dela (COBISS): Magistrsko delo/naloga
Študijski program: 0
Konec prepovedi (OpenAIRE): 1970-01-01
Komentar na gradivo: Univ. v Ljubljani, Biotehniška fak., Študij mikrobiologije
Strani: XII, 61 f., [11] f. pril.
ID: 11141143