Nina Milevoj (Avtor), Urša Lampreht Tratar (Avtor), Ana Nemec (Avtor), Andreja Brožič (Avtor), Katarina Žnidar (Avtor), Gregor Serša (Avtor), Maja Čemažar (Avtor), Nataša Tozon (Avtor)

Povzetek

The aim of this study was to evaluate the safety and efficacy of the combination of electrochemotherapy (ECT) with bleomycin and gene electrotransfer (GET) of plasmid encoding canine interleukin 12 (IL-12) for the treatment of canine oral malignant melanoma (OMM). Our focus was to determine the effect of the treatment on achieving local tumor control and stimulation of an antitumor immune response. Nine dogs with histologically confirmed OMM stage I to III were included in a prospective, non-randomized study. The dogs were treated with a combination of cytoreductive surgery, ECT and IL-12 GET, which was repeated up to five times, depending on the clinical response to the treatment, evaluated according to the follow-up protocol (7, 14 and 28 days after, the last treatment). One month after treatment, the objective response (OR) rate was 67% (6/9). Median survival time (MST) was 6 months and, even though the disease progressed in 8/9 patients at the end of the observation period (2 to 22 months), four animals were euthanized due to tumor-unrelated reasons. In addition, we observed a decline in the percentage of regulatory T cells (Treg) in the peripheral blood in the course of the treatment, which could be attributed to a systemic antitumor response to IL-12 GET. The results of this study suggest that a combination of ECT and IL-12 GET may be beneficial for dogs with OMM, especially when other treatment approaches are not acceptable due to their invasiveness or cost.

Ključne besede

electrochemotherapy;surgery;oral;interleukin-12;cytoreductive surgery;oral malignant melanoma;regulatory T cells;dogs;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL VF - Veterinarska fakulteta
Založnik: Elsevier
UDK: 636.7.09:616
COBISS: 4730490 Povezava se bo odprla v novem oknu
ISSN: 0034-5288
Št. ogledov: 784
Št. prenosov: 582
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarne ključne besede: Electrochemotherapy;Melanoma;Surgery, Oral;Interleukin-12;Dogs;
Vrsta dela (COBISS): Znanstveno delo
Konec prepovedi (OpenAIRE): 2019-11-11
Strani: str. 40-49
Zvezek: ǂVol. ǂ122
Čas izdaje: 2019
DOI: 10.1016/j.rvsc.2018.11.001
ID: 11191855