diplomsko delo
Nataša Vukadinović (Avtor), Tatjana Zupančič (Mentor)

Povzetek

Resveratrol je eden od manj znanih, vendar zelo močnih antioksidantov. Poleg tega ima še protivnetne lastnosti ter deluje estrogeno, antiagregacijsko in antikoagulativno. Najdemo ga v različnih rastlinah, največ v grozdju in jagodičevju. S procesom fermentacije in maceracije se ga nekaj ekstrahira iz grozdja v vino. V tem diplomskem delu sem uporabila metodo tekočinske kromatografije visoke ločljivosti. Prednost te tehnike je visoka ločljivost, občutljivost, ponovljivost, možnost avtomatizacije in široka možnost uporabe. Preizkusila sem dve HPLC analizni metodi za določitev vsebnosti resveratrola. Obe sem preizkušala na istem HPLC sistemu ter uporabila isto kolono, Gemini NX 150x4,6 mm - 5µm delci z ustrezno predkolono. Ker pri nobeni metodi nisem dobila ustrezne ločbe resveratrola od matriksa v vzorcu vina, sem modificirala HPLC analizno metodo. Spreminjala sem delež metanola v topilu, delež acetonitrila v mobilni fazi ter temperaturo kolone. Kolono je bilo treba zaradi obrabljenosti zamenjati z novejšo. Ko je bila dosežena ustrezna ločba resveratrola, sem metodo validirala po kriterijih validacije. Z validirano metodo sem določila resveratrol v različnih vzorcih slovenskih vin, v vzorcu prehranskega dopolnila in v soku aronije. Med rdečimi vini je imel najvišjo vsebnost resveratrola teran, nato cviček ter najnižjo rumeni muškat. V soku aronije resveratrola ni bilo zaznati.

Ključne besede

analizna kemija;analizne metode;razvoj analiznih metod;tekočinska kromatografija visoke ločljivosti;HPLC;resveratrol;določanje resveratrola;vino;validacija analizne metode;diplomske naloge;

Podatki

Jezik: Slovenski jezik
Leto izida:
Tipologija: 2.11 - Diplomsko delo
Organizacija: UL FKKT - Fakulteta za kemijo in kemijsko tehnologijo
Založnik: [N. Vukadinović]
UDK: 543.544.5.068.7(043.2)
COBISS: 1538326467 Povezava se bo odprla v novem oknu
Št. ogledov: 1093
Št. prenosov: 205
Ocena: 0 (0 glasov)
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Ostali podatki

Sekundarni jezik: Angleški jezik
Sekundarni naslov: Development and validation of HPLC method for determination of resveratrol
Sekundarni povzetek: Resveratrol is a very strong antioxidant but not very known. In addition, it has estrogenic, antiaggregation and anticoagulant properties. It is found in various plants, mostly in berries. Because of the fermentation and maceration processes some of it can be found in wine. In this diploma thesis the high performance liquid chromatography method was used. The advantage of this technique is high resolution, sensitivity, repeatability, possibility of automation and wide range of use. Two HPLC methods were tested for the resveratrol determination. Methods were tested on same HPLC system, same column, Gemini NX 150x4.6 mm - 5µm particles with the appropriate precolumn. Proper separation of resveratrol form matrix in samples of wine, was not obtained by any method, so the HPLC method was modified. The proportion of methanol in solvent, proportion of acetonitrile in mobile phase and temperature of column were changed. Due to triteness, the column had to be replaced with a new one. When an appropriate resolution of resveratrol from matrix was reached, the method was validated according to the validation criteria. After the validation, resveratrol was determined in various samples of Slovenian wines, in a dietary supplement and in aronia juice. The highest content of resveratrol in red wines was determined in teran, followed by cviček and the lowest in yellow muscat. Resveratrol was not detected in aronia juice.
Sekundarne ključne besede: resveratrol;wine;HPLC;validation;
Vrsta dela (COBISS): Diplomsko delo/naloga
Študijski program: 1000374
Konec prepovedi (OpenAIRE): 1970-01-01
Komentar na gradivo: Univ. v Ljubljani, Fak. za kemijo in kemijsko tehnologijo, VSŠ Kemijska tehnologija
Strani: 38 str.
ID: 11217772