Brian R. Graziano (Avtor), Jason P. Town (Avtor), Ewa Sitarska (Avtor), Tamas L. Nagy (Avtor), Miha Fošnarič (Avtor), Samo Penič (Avtor), Aleš Iglič (Avtor), Veronika Kralj-Iglič (Avtor), Nir S. Gov (Avtor), Alba Diz-Muñoz (Avtor), Orion D. Weiner (Avtor)

Povzetek

Migratory cells use distinct motility modes to navigate different microenvironments, but it is unclear whether these modes rely on the same core set of polarity components. To investigate this, we disrupted actin-related protein 2/3 (Arp2/3) and the WASP-family verprolin homologous protein (WAVE) complex, which assemble branched actin networks that are essential for neutrophil polarity and motility in standard adherent conditions. Surprisingly, confinement rescues polarity and movement of neutrophils lacking these components, revealing a processive bleb-based protrusion program that is mechanistically distinct from the branched actin-based protrusion program but shares some of the same core components and underlying molecular logic. We further find that the restriction of protrusion growth to one site does not always respond to membrane tension directly, as previously thought, but may rely on closely linked properties such as local membrane curvature. Our work reveals a hidden circuit for neutrophil polarity and indicates that cells have distinct molecular mechanisms for polarization that dominate in different microenvironments.

Ključne besede

branched actin networks;neutrophil polarity;neutrophil motility;adherent conditions;membrane tension;cell confinement;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Organizacija: UL ZF - Zdravstvena fakulteta
UDK: 577
COBISS: 5728875 Povezava se bo odprla v novem oknu
ISSN: 1545-7885
Št. ogledov: 708
Št. prenosov: 440
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Vrsta dela (COBISS): Članek v reviji
Strani: str. 1-34
Letnik: ǂVol. ǂ17
Zvezek: ǂiss. ǂ10
Čas izdaje: 2019
DOI: 10.1371/journal.pbio.3000457
ID: 11324698