ǂan ǂupdate
Tomasz Jagielski (Avtor), Paulina Borówka (Avtor), Zofia Bakuła (Avtor), Jakub Lach (Avtor), Błażej Marciniak (Avtor), Anna Brzostek (Avtor), Jarosław Dziadek (Avtor), Mikołaj Dziurzyński (Avtor), Lian Pennings (Avtor), Jakobus van Ingen (Avtor), Marija Žolnir-Dovč (Avtor), Dominik Strapagiel (Avtor)

Povzetek

Only very recently, has it been proposed that the hitherto existing Mycobacteriumkansasii subtypes (I–VI) should be elevated, each, to a species rank. Consequently, the former M. kansasii subtypes have been denominated as Mycobacterium kansasii (former type I), Mycobacterium persicum (II), Mycobacterium pseudokansasii (III), Mycobacterium innocens (V), and Mycobacterium attenuatum (VI). The present work extends the recently published findings by using a three-pronged computational strategy, based on the alignment fraction-average nucleotide identity, genome-to-genome distance, and core-genome phylogeny, yet essentially independent and much larger sample, and thus delivers a more refined and complete picture of the M. kansasii complex. Furthermore, five canonical taxonomic markers were used, i.e., 16S rRNA, hsp65, rpoB, and tuf genes, as well as the 16S-23S rRNA intergenic spacer region (ITS). The three major methods produced highly concordant results, corroborating the view that each M. kansasii subtype does represent a distinct species. This work not only consolidates the position of five of the currently erected species, but also provides a description of the sixth one, i.e., Mycobacterium ostraviense sp. nov. to replace the former subtype IV. By showing a close genetic relatedness, amonophyletic origin, and overlapping phenotypes, our findings support the recognition of the M. kansasii complex (MKC), accommodating all M. kansasii-derived species and Mycobacterium gastri. None of the most commonly used taxonomic markers was shown to accurately distinguish all the MKC species. Likewise, no species-specific phenotypic characteristics were found allowing for species differentiation within the complex, except the non-photochromogenicity of M. gastri. To distinguish, most reliably, between the MKC species, and between M. kansasii and M. persicum in particular, whole-genome-based approaches should be applied. In the absence of clear differences in the distribution of the virulence-associated region of difference 1 genes among the M. kansasii-derived species, the pathogenic potential of each of these species can only be speculatively assessed based on their prevalence among the clinically relevant population. Large-scale molecular epidemiological studies are needed to provide a better understanding of the clinical significance and pathobiology of the MKC species. The results of the in vitro drug susceptibility profiling emphasize the priority of rifampicin administration in the treatment of MKC-induced infections, while undermining the use of ethambutol, due to a high resistance to this drug.

Ključne besede

kompleks Mycobacterium kansasii;Mycobacterium ostraviense sp. nov.;taksonomija;Mycobacterium kansasii complex;taxonomy;

Podatki

Jezik: Angleški jezik
Leto izida:
Tipologija: 1.01 - Izvirni znanstveni članek
Založnik: Frontiers Media SA
UDK: 579
COBISS: 2048616049 Povezava se bo odprla v novem oknu
ISSN: 1664-302X
Št. ogledov: 1208
Št. prenosov: 806
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarne ključne besede: Nontuberculous mycobacteria;Genetics;Mycobacterium kansasii;Whole genome sequencing;Classification;Microbiology;Netuberkulozne mikobakterije;Genetika;Sekvenciranje celotnega genoma;Klasifikacija;Mikrobiologija;
Komentar vira: Nasl. z nasl. zaslona; Soavtorica iz Slovenije: Manca Žolnir-Dovč; Opis vira z dne 17. 1. 2020; Št. prispevka: 2918;
Strani: str. 1-27
Zvezek: ǂVol. ǂ10
Čas izdaje: 15 Jan. 2020
DOI: 10.3389/fmicb.2019.02918
ID: 11928337