diplomsko delo
Sara Andrejka (Avtor), Tomaž Lampe (Recenzent), Klemen Bohinc (Mentor), Anamarija Zore (Komentor)

Povzetek

Uvod: Ortoze so rigidni, polrigidni ali mehki pripomočki, pritrjeni na del telesa. Pod rigidnim delom ortoze je nameščen tekstilni material, zaradi katerega je nošenje ortoze bolj udobno. Ti materiali so mehki in so v stalnem stiku s kožo, zato je mogoča adhezija bakterij iz kožne mikrobiote. Človeška koža je gosto naseljena z mikroorganizmi, ki predstavljajo našo mikrobioto. Adhezija bakterij na površine materialov je pogojena s številnimi dejavniki, kot so zeta potencial, hrapavost in hidrofobnost materiala. Prav tako na adhezijo vplivajo karakteristike bakterij. Namen: Ugotoviti, koliko in kakšne bakterije se nahajajo v naravni mikrobioti kože. Ugotoviti, kako zeta potencial materialov in njihova hidrofobnost vplivajo na adhezijo bakterij, ki se nahajajo na površini kože. Rezultate med seboj primerjati in ugotoviti, ali je kateri izmed petih materialov bolj odporen proti adheziji bakterij. Rezultate primerjati z ugotovitvami v drugi strokovni literaturi. Metode dela: V diplomskem delu smo uporabili pet materialov, ki se uporabljajo pri izdelavi ortoz: Neopren, Najlon, Lycra, xDRY in bambusova vlakna. Pri teh materialih smo izmerili zeta potencial, določili mejni kot in s tem ugotovili njihovo hidrofobnost. V treh poskusih smo materiale nalepili na tri različna mesta na koži. Na teh mestih smo odvzeli tudi mokri bris. Materiale smo pustili nalepljene 12 ur in jih nato za 30 sekund odtisnili v krvni agar. Vzorce smo inkubirali 24 ur pri 37 °C ter nato prešteli kolonije bakterij. Rezultati: Največji zeta potencial smo izmerili na bambusovih vlaknih, nekoliko manjši zeta potencial sta imela Najlon in Lycra, najbolj negativen potencial pa xDRY in Neopren. Neopren in xDRY sta hidrofobna materiala, pri slednjem je bil izmerjen nekoliko večji mejni kot. Za ostale materiale pa smo določili, kako hitro vpijejo kapljico vode. Bambusova vlakna so najbolj hidrofilen material, sledita Najlon in Lycra. Število adheziranih bakterij je bilo od 0 do 2 koloniji na posameznem nanosu. Razprava in sklep: Na vse materiale se je v povprečju adhezirala manj kot ena kolonija bakterij. Najmanj adheziranih bakterij je bilo na Neoprenu, ki je imel tudi najbolj negativen zeta potencial. Največje število bakterij pa se je adheziralo na Najlon. V primerjavi s številom kolonij pri mokrem brisu pa je število kolonij na vseh materialih ostalo nizko. Iz dobljenih rezultatov lahko sklepamo, da so materiali primerni za uporabo v ortotiki.

Ključne besede

diplomska dela;ortotika in protetika;adhezija bakterij;naravna mikrobiota kože;ortoza;tekstilni material;hidrofobnost;zeta potencial;

Podatki

Jezik: Slovenski jezik
Leto izida:
Tipologija: 2.11 - Diplomsko delo
Organizacija: UL ZF - Zdravstvena fakulteta
Založnik: [S. Andrejka]
UDK: 617.3
COBISS: 51955971 Povezava se bo odprla v novem oknu
Št. ogledov: 439
Št. prenosov: 87
Ocena: 0 (0 glasov)
Metapodatki: JSON JSON-RDF JSON-LD TURTLE N-TRIPLES XML RDFA MICRODATA DC-XML DC-RDF RDF

Ostali podatki

Sekundarni jezik: Angleški jezik
Sekundarni naslov: Bacterial adhesion to textile materials used in orthopedic devices
Sekundarni povzetek: Introduction: Orthoses are rigid, semi-rigid or soft devices, attached to external part of the body. Under the rigid part of orthoses there is a textile material, which makes wearing of the orthosis more comfortable. These materials are in constant contact with the skin, that makes them more exposed for bacterial adhesion from skin microbiota. Microorganisms that inhabit human skin represent our natural microbiota. Bacterial adhesion to the surfaces of materials is conditioned by a number of factors such as: zeta potencial, roughness and hydrophobicity of the material and is also affected by the characteristics of the bacterias. Purpose: Determine how many and what kind of bacterias are found in the natural skin microbiota. To determine how zeta potencial and hydrophobicity of the materials affect adhesion of bacterial from the surface of the skin. Comparing results and decide which of five materials is more resistant to bacterial adhesion and compare those results with other literature. Methods: Five different materials that are used in production of orthoses, were used for the research: Neoprene, Nylon, Lycra, xDRY and Bamboo Fibers. We measured zeta potencial and determined hydrophobcitiy for these materials, which were then attached to three different places on the skin within three different experiments. On the same places we also took a wet swab. Materials were attached for 12 hours and then imprinted in blood agar for 30 seconds. The samples were incubated for 24 hours at 37°C, after that bacterial colonies were counted.Results: The highest zeta potencial was on Bamboo fibers, followed by Nylon, Lycra, xDRY and the lowest potencial was measured on Neoprene. Neoprene and xDRY are hydrophobic materials, where xDRY has a higher contact angle. Other three materials are hydrophilic, so we determined how quckly the materials are absorbing a drop of water. The most hydrophilic materials is Bamboo fiber, next is Nylon and the least hydrophilic is Lycra. The avarage number of adhered bacterias ranged from 0 to 2 colonies per application Discussion and conclusion: On average, less than one colony of bacteria adhered to every material. The number of adhered bacterias was the lowest on Neopren, which also had the most negative zeta potencial. And the largest number of adheres bacterias was on Nylon. However the number of colonies on all materials remained low in comparison with the number of colonies on wet swabs. From the obtained results we can conclude that the materials are sutable for use in orthotics.
Sekundarne ključne besede: diploma theses;orthotics and prosthetics;bacterial adhesion;skin microbiota;orthoses;textile materials;hydrophobicity;zeta potential;
Vrsta dela (COBISS): Diplomsko delo/naloga
Študijski program: 0
Komentar na gradivo: Univ. v Ljubljani, Zdravstvena fak., Oddelek za protetiko
Strani: 39 str.
ID: 12565943